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'Light-Guided Sectioning' for Precise Localization and 3D Visualization of In Situ Optical Implants

40 Pages Posted: 7 Dec 2020 Publication Status: Published

See all articles by Anat Kahan

Anat Kahan

California Institute of Technology - Division of Biology and Biological Engineering

Alon Greenbaum

North Carolina State University - NC State/UNC Joint Department of Biomedical Engineering; California Institute of Technology - Division of Biology and Biological Engineering

Min J. Jang

California Institute of Technology - Division of Biology and Biological Engineering

J. Elliott Robinson

California Institute of Technology - Division of Biology and Biological Engineering

Jounhong Ryan Cho

California Institute of Technology - Division of Biology and Biological Engineering

Xinhong Chen

California Institute of Technology - Division of Biology and Biological Engineering

Pegah Kassraian

California Institute of Technology - Division of Biology and Biological Engineering

Daniel A. Wagenaar

California Institute of Technology - Division of Biology and Biological Engineering

Viviana Gradinaru

California Institute of Technology - Division of Biology and Biological Engineering

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Abstract

In the last two decades, there has been a significant increase in the use of implanted optical devices to control and monitor neuronal activity in vivo. However, post hoc examination of the implant location is commonly achieved via 2D histology, which often suffers from distortion and loss during tissue processing. To address this, we developed a 3D method that allows post hoc labeling and clearing of tissue that preserves the size of the tissue while leaving the optical implant unperturbed. By coupling the implant to a light-emitting diode, the implant itself can serve as a light guide for removal of redundant tissue (light-guided sectioning, LiGS). The residual tissue, including the tissue–implant interface, is then processed as a whole for further investigation. This enables the precise location of the optical implant to be determined as well as the identification of tissue changes at the implant site. LiGS can also be used for accurate cell registration of ex vivo histology with single-cell two-photon calcium images obtained through gradient-index (GRIN) lenses. We anticipate that LiGS will provide valuable additional information in any experiment that uses optical implants and will increase reproducibility through well-defined fiber-to-target localization.

Suggested Citation

Kahan, Anat and Greenbaum, Alon and Jang, Min J. and Robinson, J. Elliott and Cho, Jounhong Ryan and Chen, Xinhong and Kassraian, Pegah and Wagenaar, Daniel A. and Gradinaru, Viviana, 'Light-Guided Sectioning' for Precise Localization and 3D Visualization of In Situ Optical Implants. Available at SSRN: https://ssrn.com/abstract=3744424 or http://dx.doi.org/10.2139/ssrn.3744424
This version of the paper has not been formally peer reviewed.

Anat Kahan

California Institute of Technology - Division of Biology and Biological Engineering ( email )

Pasadena, CA 91125
United States

Alon Greenbaum

North Carolina State University - NC State/UNC Joint Department of Biomedical Engineering ( email )

NC
United States

California Institute of Technology - Division of Biology and Biological Engineering ( email )

Pasadena, CA 91125
United States

Min J. Jang

California Institute of Technology - Division of Biology and Biological Engineering ( email )

Pasadena, CA 91125
United States

J. Elliott Robinson

California Institute of Technology - Division of Biology and Biological Engineering ( email )

Pasadena, CA 91125
United States

Jounhong Ryan Cho

California Institute of Technology - Division of Biology and Biological Engineering

Pasadena, CA 91125
United States

Xinhong Chen

California Institute of Technology - Division of Biology and Biological Engineering ( email )

Pasadena, CA 91125
United States

Pegah Kassraian

California Institute of Technology - Division of Biology and Biological Engineering ( email )

Pasadena, CA 91125
United States

Daniel A. Wagenaar

California Institute of Technology - Division of Biology and Biological Engineering ( email )

Pasadena, CA 91125
United States

Viviana Gradinaru (Contact Author)

California Institute of Technology - Division of Biology and Biological Engineering ( email )

Pasadena, CA 91125
United States

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