Cochlear Implant-Based Electric-Acoustic Stimulation Modulates Neural Stem Cell-Derived Neural Regeneration

35 Pages Posted: 7 Jan 2021

See all articles by Rongrong Guo

Rongrong Guo

Soochow University - Institute for Cardiovascular Science & Department of Cardiovascular Surgery

Menghui Liao

Southeast University - Key Laboratory for Developmental Genes and Human Disease

Xiaofeng Ma

Nanjing Medical University - Department of Otorhinolaryngology-Head and Neck Surgery

Yangnan Hu

Southeast University - Key Laboratory for Developmental Genes and Human Disease

Xiaoyun Qian

Nanjing Medical University - Department of Otorhinolaryngology-Head and Neck Surgery

Miao Xiao

Soochow University - Institute for Cardiovascular Science & Department of Cardiovascular Surgery

Xia Gao

Nanjing Medical University - Department of Otorhinolaryngology-Head and Neck Surgery

Renjie Chai

Southeast University - Key Laboratory for Developmental Genes and Human Disease; Southeast University - Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research and School of Chemistry and Chemical Engineering

Mingliang Tang

Soochow University - Institute for Cardiovascular Science

Abstract

Cochlear implantation is considered to be the best therapeutic method for profound sensorineural hearing loss, but insufficient numbers of functional spiral ganglion neurons hinder the clinical effects of cochlear implantation. Stem cell transplantation has the potential to provide novel strategies for spiral ganglion neuron regeneration after injury. However, some obstacles still need to be overcome, such as low survival and uncontrolled differentiation. Several novel technologies show promise for modulating neural stem cell behaviors to address these issues. Here, a device capable of electrical stimulation was designed by combining a cochlear implant with a graphene substrate. Neural stem cells (NSCs) were cultured on the graphene substrate and subjected to electrical stimulation transduced from sound waves detected by the cochlear implant. Cell behaviors were studied, and this device showed good biocompatibility for NSCs. More importantly, electric-acoustic stimulation with higher frequencies and amplitudes induced NSC death and apoptosis, and electric-acoustic stimulation could promote NSCs to proliferate and differentiate into neurons only when low-frequency stimulation was supplied. The present study provides experimental evidence for understanding the regulatory role of electric-acoustic stimulation on NSCs and highlights the potentials of the above-mentioned device in stem cell therapy for hearing loss treatment.

Keywords: electric-acoustic stimulation; neural stem cells; graphene; differentiation

Suggested Citation

Guo, Rongrong and Liao, Menghui and Ma, Xiaofeng and Hu, Yangnan and Qian, Xiaoyun and Xiao, Miao and Gao, Xia and Chai, Renjie and Tang, Mingliang, Cochlear Implant-Based Electric-Acoustic Stimulation Modulates Neural Stem Cell-Derived Neural Regeneration. Available at SSRN: https://ssrn.com/abstract=3762217 or http://dx.doi.org/10.2139/ssrn.3762217

Rongrong Guo

Soochow University - Institute for Cardiovascular Science & Department of Cardiovascular Surgery

No. 1 Shizi Street
Taipei, Jiangsu 215006
Taiwan

Menghui Liao

Southeast University - Key Laboratory for Developmental Genes and Human Disease

Xiaofeng Ma

Nanjing Medical University - Department of Otorhinolaryngology-Head and Neck Surgery

300 Guangzhou Road
Nanjing, 210029
China

Yangnan Hu

Southeast University - Key Laboratory for Developmental Genes and Human Disease

Xiaoyun Qian

Nanjing Medical University - Department of Otorhinolaryngology-Head and Neck Surgery

Nanjing
China

Miao Xiao

Soochow University - Institute for Cardiovascular Science & Department of Cardiovascular Surgery

Xia Gao

Nanjing Medical University - Department of Otorhinolaryngology-Head and Neck Surgery

300 Guangzhou Road
Nanjing, Jiangsu 210029
China

Renjie Chai

Southeast University - Key Laboratory for Developmental Genes and Human Disease ( email )

Sipailou 2#
Nanjing, Jiangsu Province 210096
China

Southeast University - Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research and School of Chemistry and Chemical Engineering ( email )

Mingliang Tang (Contact Author)

Soochow University - Institute for Cardiovascular Science ( email )

Suzhou
China

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