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Bottom-Up Inputs are Required for the Establishment of Top-Down Connectivity Onto Cortical Layer 1 Neurogliaform Cells

47 Pages Posted: 18 Feb 2021 Publication Status: Published

See all articles by Leena A. Ibrahim

Leena A. Ibrahim

Harvard Medical School - Department of Neurobiology

Shuhan Huang

Harvard Medical School - Department of Neurobiology

Marian Fernández-Otero

Harvard Medical School - Department of Neurobiology

Mia Sherer

Northeastern University

Spurti Vemuri

Northeastern University

Qing Xu

New York University Abu Dhabi - Center for Genomics & Systems Biology

Robert Machold

New York University (NYU) - NYU Langone Medical Center

Bernardo Rudy

New York University (NYU) - Neuroscience Institute

Gord Fishell

Harvard Medical School - Department of Neurobiology

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Abstract

Higher order feedback projections to sensory cortical areas converge on layer 1 (L1), the primary site for integration of top-down information via the apical dendrites of pyramidal neurons and L1 GABAergic interneurons. Here, we investigated the contribution of early thalamic inputs onto L1 interneurons for the establishment of top-down inputs in the primary visual cortex. We find that bottom-up thalamic inputs predominate during early L1 development and preferentially target neurogliaform cells. We find that these projections are critical for the subsequent strengthening of feedback inputs from the anterior cingulate cortex. Enucleation or selective removal of thalamic afferents blocked this phenomenon. Notably, while early activation of anterior cingulate afferents resulted in a premature strengthening of these top-down inputs to neurogliaform cells, this was also dependent on thalamic inputs. Our results demonstrate that the proper establishment of top-down feedback inputs critically depends on bottom-up inputs from the thalamus during early postnatal development.

Suggested Citation

Ibrahim, Leena A. and Huang, Shuhan and Fernández-Otero, Marian and Sherer, Mia and Vemuri, Spurti and Xu, Qing and Machold, Robert and Rudy, Bernardo and Fishell, Gord, Bottom-Up Inputs are Required for the Establishment of Top-Down Connectivity Onto Cortical Layer 1 Neurogliaform Cells. Available at SSRN: https://ssrn.com/abstract=3787895 or http://dx.doi.org/10.2139/ssrn.3787895
This version of the paper has not been formally peer reviewed.

Leena A. Ibrahim

Harvard Medical School - Department of Neurobiology ( email )

220 Longwood Avenue Goldenson Building, Room 420
Boston, MA
United States

Shuhan Huang

Harvard Medical School - Department of Neurobiology

220 Longwood Avenue Goldenson Building, Room 420
Boston, MA
United States

Marian Fernández-Otero

Harvard Medical School - Department of Neurobiology ( email )

220 Longwood Avenue Goldenson Building, Room 420
Boston, MA
United States

Mia Sherer

Northeastern University ( email )

220 B RP
Boston, MA 02115
United States

Spurti Vemuri

Northeastern University ( email )

220 B RP
Boston, MA 02115
United States

Qing Xu

New York University Abu Dhabi - Center for Genomics & Systems Biology

PO Box 129188
Abu Dhabi
United Arab Emirates

Robert Machold

New York University (NYU) - NYU Langone Medical Center ( email )

NY
United States

Bernardo Rudy

New York University (NYU) - Neuroscience Institute

Bobst Library, E-resource Acquisitions
20 Cooper Square 3rd Floor
New York, NY 10003-711
United States

Gord Fishell (Contact Author)

Harvard Medical School - Department of Neurobiology ( email )

220 Longwood Avenue Goldenson Building, Room 420
Boston, MA
United States

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