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Levels of SARS-CoV-2 Lineage P.1 Neutralization by Antibodies Elicited after Natural Infection and Vaccination

24 Pages Posted: 1 Mar 2021

See all articles by William M. de Souza

William M. de Souza

Virology Research Centre, Ribeirão Preto Medical School, University of São Paulo

Mariene R. Amorim

Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas

Renata Sesti-Costa

National Biosciences Laboratory, Brazilian Centre for Research in Energy and Materials (CNPEM)

Lais D. Coimbra

National Biosciences Laboratory, Brazilian Centre for Research in Energy and Materials (CNPEM)

Daniel Augusto de Toledo-Teixeira

Department of Genetics, Evolution, Microbiology and Immunology

Pierina L. Parise

Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas

Priscilla P. Barbosa

Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas

Karina Bispo-dos-Santos

Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas

Luciana S. Mofatto

Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas

Camila L. Simeoni

Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas

Natalia S. Brunetti

Autoimmune Research Laboratory, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas

Ingra M. Claro

Tropical Medicine Institute, Medical School, University of São Paulo

Adriana S. S. Duarte

Hematology and Transfusion Medicine Centre, University of Campinas

Thais M. Coletti

Tropical Medicine Institute, Medical School, University of São Paulo

Audrey B. Zangirolami

Hematology and Transfusion Medicine Centre, University of Campinas

Carolina Costa-Lima

Hematology and Transfusion Medicine Centre, University of Campinas

Arilson Bernardo S. P. Gomes

Autoimmune Research Laboratory, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas

Lucas I. Buscaratti

Brazilian Laboratory on Silencing Technologies, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas

Flavia C. Sales

Tropical Medicine Institute, Medical School, University of São Paulo

Vitor A. Costa

Hematology and Transfusion Medicine Centre, University of Campinas

Lucas A.M. Franco

Tropical Medicine Institute, Medical School, University of São Paulo

Darlan S. Candido

University of Oxford

Oliver G. Pybus

University of Oxford

Jaqueline G. de Jesus

Tropical Medicine Institute, Medical School, University of São Paulo

Camila A. M. Silva

Tropical Medicine Institute, Medical School, University of São Paulo

Mariana S. Ramundo

Tropical Medicine Institute, Medical School, University of São Paulo

Giulia M. Ferreira

Tropical Medicine Institute, Medical School, University of São Paulo

Mariana C. Pinho

Tropical Medicine Institute, Medical School, University of São Paulo

Leandro M. Souza

Tropical Medicine Institute, Medical School, University of São Paulo

Esmenia C. Rocha

Tropical Medicine Institute, Medical School, University of São Paulo

Pamela S. Andrade

Tropical Medicine Institute, Medical School, University of São Paulo

Myuki A.E. Crispim

Hemoam - Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas

Grazielle C. Maktura

Brazilian Laboratory on Silencing Technologies, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas

Erika R. Manuli

Tropical Medicine Institute, Medical School, University of São Paulo

Magnun N.N. Santos

Department of Clinical Pathology, School of Medical Sciences, University of Campinas

Cecilia C. Camilo

CDL Laboratório Santos e Vidal Ltda.

Rodrigo N. Angerami

Department of Internal Medicine, School of Medical Sciences, University of Campinas

Maria L. Moretti

Department of Internal Medicine, School of Medical Sciences, University of Campinas

Fernando R. Spilki

One Health Laboratory, Feevale University

Clarice W. Arns

Animal Virology Laboratory, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas,

Marcelo Addas-Carvalho

Hematology and Transfusion Medicine Centre, University of Campinas

Bruno D. Benites

Hematology and Transfusion Medicine Centre, University of Campinas

Marcelo A. Mori

Universidade Estadual de Campinas (UNICAMP) - Institute of Biology

Nelson Gaburo

DB Diagnósticos do Brasil

Christopher Dye

Department of Zoology, University of Oxford

Chieh-Hsi Wu

University of Southampton

Henrique Marques-Souza

Brazilian Laboratory on Silencing Technologies, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas

Rafael E. Marques

National Biosciences Laboratory, Brazilian Centre for Research in Energy and Materials (CNPEM)

Alessandro Farias

Autoimmune Research Laboratory, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas

Michael S. Diamond

Washington University in St. Louis - Department of Medicine

Nuno R. Faria

Imperial College London

Ester C. Sabino

University of São Paulo (USP) - Institute of Tropical Medicine

Fabiana Granja

Universidade Estadual de Campinas (UNICAMP) - Laboratory of Emerging Viruses

Jose Luiz Proenca-Modena

Universidade Estadual de Campinas (UNICAMP) - Laboratory of Emerging Viruses

More...

Abstract

Background: A new SARS-CoV-2 lineage, named P.1 (20J/501Y.V3), has recently been detected in Brazil. Mutations accrued by the P.1 lineage include amino acid changes in the receptor-binding domain of the spike protein that also are reported in variants of concern in the United Kingdom (B.1.1.7) and South Africa (B.1.325).

Methods: We isolated two P.1-containing specimens from nasopharyngeal and bronchoalveolar lavage samples of patients of Manaus, Brazil. We measured neutralization of the P.1 virus after incubation with the plasma of 19 COVID-19 convalescent blood donors and recipients of the chemically-inactivated CoronaVac vaccine and compared these results to neutralization of a SARS-CoV-2 B-lineage previously circulating in Brazil.

Findings: The immune plasma of COVID-19 convalescent blood donors had 6-fold less neutralizing capacity against the P.1 than against the B-lineage. Moreover, five months after booster immunization with CoronaVac, plasma from vaccinated individuals failed to efficiently neutralize P.1 lineage isolates.

Interpretation: These data indicate that the P.1 lineage may escape from neutralizing antibodies generated in response to polyclonal stimulation against previously circulating variants of SARS-CoV-2.

Funding: São Paulo Research Foundation, MCTI/FINEP, Medical Research Council, National Council for Scientific and Technological Development, National Institutes of Health.

Conflict of Interest: M.S.D. is a consultant for Inbios, Vir Biotechnology, NGMBiopharmaceuticals, and Carnival Corporation, and on the Scientific Advisory Boards of Moderna and Immunome. The Diamond laboratory has received funding support in sponsored research agreements from Moderna, Vir Biotechnology, and Emergent BioSolutions.

Ethical Approval: All procedures followed the ethical standards of the responsible committee on humanexperimentation and approved by the ethics committees from the University of Campinas, Brazil (Approval number CONEP 4.021.484 for plasma collection of blood donors, CAEE32078620.4.0000.5404 and 30227920.9.0000.5404 for the sampling of vaccinated and viral genome sequencing, respectively). All patient data were anonymized before study inclusion.Informed consent was obtained from all subjects for being included in the study.

Suggested Citation

de Souza, William M. and Amorim, Mariene R. and Sesti-Costa, Renata and Coimbra, Lais D. and Toledo-Teixeira, Daniel Augusto de and Parise, Pierina L. and Barbosa, Priscilla P. and Bispo-dos-Santos, Karina and Mofatto, Luciana S. and Simeoni, Camila L. and Brunetti, Natalia S. and Claro, Ingra M. and Duarte, Adriana S. S. and Coletti, Thais M. and Zangirolami, Audrey B. and Costa-Lima, Carolina and Gomes, Arilson Bernardo S. P. and Buscaratti, Lucas I. and Sales, Flavia C. and Costa, Vitor A. and Franco, Lucas A.M. and Candido, Darlan S. and Pybus, Oliver G. and de Jesus, Jaqueline G. and Silva, Camila A. M. and Ramundo, Mariana S. and Ferreira, Giulia M. and Pinho, Mariana C. and Souza, Leandro M. and Rocha, Esmenia C. and Andrade, Pamela S. and Crispim, Myuki A.E. and Maktura, Grazielle C. and Manuli, Erika R. and Santos, Magnun N.N. and Camilo, Cecilia C. and Angerami, Rodrigo N. and Moretti, Maria L. and Spilki, Fernando R. and Arns, Clarice W. and Addas-Carvalho, Marcelo and Benites, Bruno D. and Mori, Marcelo A. and Gaburo, Nelson and Dye, Christopher and Wu, Chieh-Hsi and Marques-Souza, Henrique and Marques, Rafael E. and Farias, Alessandro and Diamond, Michael S. and Faria, Nuno R. and Sabino, Ester C. and Granja, Fabiana and Proenca-Modena, Jose Luiz, Levels of SARS-CoV-2 Lineage P.1 Neutralization by Antibodies Elicited after Natural Infection and Vaccination. Available at SSRN: https://ssrn.com/abstract=3793486 or http://dx.doi.org/10.2139/ssrn.3793486

William M. De Souza

Virology Research Centre, Ribeirão Preto Medical School, University of São Paulo ( email )

Av. Prof. Luciano Gualberto 380
Sao Paulo, 05508-010
Brazil

Mariene R. Amorim

Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Renata Sesti-Costa

National Biosciences Laboratory, Brazilian Centre for Research in Energy and Materials (CNPEM) ( email )

Campinas
Sao Paulo, 13083-970
Brazil

Lais D. Coimbra

National Biosciences Laboratory, Brazilian Centre for Research in Energy and Materials (CNPEM) ( email )

Campinas
Sao Paulo, 13083-970
Brazil

Daniel Augusto de Toledo-Teixeira

Department of Genetics, Evolution, Microbiology and Immunology ( email )

Monteiro Lobato St 255
Campinas, 13083-862
Brazil

Pierina L. Parise

Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Priscilla P. Barbosa

Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Karina Bispo-dos-Santos

Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Luciana S. Mofatto

Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Camila L. Simeoni

Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Natalia S. Brunetti

Autoimmune Research Laboratory, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Ingra M. Claro

Tropical Medicine Institute, Medical School, University of São Paulo ( email )

Rua Luciano Gualberto, 315
São Paulo, São Paulo 14800-901
Brazil

Adriana S. S. Duarte

Hematology and Transfusion Medicine Centre, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Thais M. Coletti

Tropical Medicine Institute, Medical School, University of São Paulo ( email )

Rua Luciano Gualberto, 315
São Paulo, São Paulo 14800-901
Brazil

Audrey B. Zangirolami

Hematology and Transfusion Medicine Centre, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Carolina Costa-Lima

Hematology and Transfusion Medicine Centre, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Arilson Bernardo S. P. Gomes

Autoimmune Research Laboratory, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Lucas I. Buscaratti

Brazilian Laboratory on Silencing Technologies, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Flavia C. Sales

Tropical Medicine Institute, Medical School, University of São Paulo

Av. Prof. Luciano Gualberto 380
Sao Paulo, 05508-010
Brazil

Vitor A. Costa

Hematology and Transfusion Medicine Centre, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Lucas A.M. Franco

Tropical Medicine Institute, Medical School, University of São Paulo ( email )

Rua Luciano Gualberto, 315
São Paulo, São Paulo 14800-901
Brazil

Darlan S. Candido

University of Oxford

Mansfield Road
Oxford, OX1 4AU
United Kingdom

Oliver G. Pybus

University of Oxford

Mansfield Road
Oxford, OX1 4AU
United Kingdom

Jaqueline G. De Jesus

Tropical Medicine Institute, Medical School, University of São Paulo ( email )

Rua Luciano Gualberto, 315
São Paulo, São Paulo 14800-901
Brazil

Camila A. M. Silva

Tropical Medicine Institute, Medical School, University of São Paulo ( email )

Rua Luciano Gualberto, 315
São Paulo, São Paulo 14800-901
Brazil

Mariana S. Ramundo

Tropical Medicine Institute, Medical School, University of São Paulo ( email )

Rua Luciano Gualberto, 315
São Paulo, São Paulo 14800-901
Brazil

Giulia M. Ferreira

Tropical Medicine Institute, Medical School, University of São Paulo ( email )

Rua Luciano Gualberto, 315
São Paulo, São Paulo 14800-901
Brazil

Mariana C. Pinho

Tropical Medicine Institute, Medical School, University of São Paulo ( email )

Rua Luciano Gualberto, 315
São Paulo, São Paulo 14800-901
Brazil

Leandro M. Souza

Tropical Medicine Institute, Medical School, University of São Paulo ( email )

Rua Luciano Gualberto, 315
São Paulo, São Paulo 14800-901
Brazil

Esmenia C. Rocha

Tropical Medicine Institute, Medical School, University of São Paulo ( email )

Rua Luciano Gualberto, 315
São Paulo, São Paulo 14800-901
Brazil

Pamela S. Andrade

Tropical Medicine Institute, Medical School, University of São Paulo ( email )

Av. Prof. Luciano Gualberto 380
Sao Paulo, 05508-010
Brazil

Myuki A.E. Crispim

Hemoam - Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas ( email )

Manaus
Brazil

Grazielle C. Maktura

Brazilian Laboratory on Silencing Technologies, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Erika R. Manuli

Tropical Medicine Institute, Medical School, University of São Paulo ( email )

Av. Prof. Luciano Gualberto 380
Sao Paulo, 05508-010
Brazil

Magnun N.N. Santos

Department of Clinical Pathology, School of Medical Sciences, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Cecilia C. Camilo

CDL Laboratório Santos e Vidal Ltda. ( email )

Manaus
Brazil

Rodrigo N. Angerami

Department of Internal Medicine, School of Medical Sciences, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Maria L. Moretti

Department of Internal Medicine, School of Medical Sciences, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Fernando R. Spilki

One Health Laboratory, Feevale University ( email )

Av. Dr. Maurício Cardoso, 510
Bairro Hamburgo Velho
Novo Hamburgo, 93510-250
Brazil

Clarice W. Arns

Animal Virology Laboratory, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas, ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Marcelo Addas-Carvalho

Hematology and Transfusion Medicine Centre, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Bruno D. Benites

Hematology and Transfusion Medicine Centre, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Marcelo A. Mori

Universidade Estadual de Campinas (UNICAMP) - Institute of Biology ( email )

Campinas
Brazil

Nelson Gaburo

DB Diagnósticos do Brasil ( email )

Sao Paulo
Brazil

Christopher Dye

Department of Zoology, University of Oxford

Mansfield Road
Oxford, Oxfordshire OX1 4AU
United Kingdom

Chieh-Hsi Wu

University of Southampton

University Rd.
Southampton SO17 1BJ, SO17 1LP
United Kingdom

Henrique Marques-Souza

Brazilian Laboratory on Silencing Technologies, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Rafael E. Marques

National Biosciences Laboratory, Brazilian Centre for Research in Energy and Materials (CNPEM) ( email )

Campinas
Sao Paulo, 13083-970
Brazil

Alessandro Farias

Autoimmune Research Laboratory, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas ( email )

Cidade Universitária Zeferino Vaz - Barão
Geraldo
Campinas - SP, 13083-970
Brazil

Michael S. Diamond

Washington University in St. Louis - Department of Medicine ( email )

St. Louis, MO 63110
United States

Nuno R. Faria

Imperial College London

Ester C. Sabino

University of São Paulo (USP) - Institute of Tropical Medicine ( email )

São Paulo
Brazil

Fabiana Granja

Universidade Estadual de Campinas (UNICAMP) - Laboratory of Emerging Viruses ( email )

Campinas
Brazil

Jose Luiz Proenca-Modena (Contact Author)

Universidade Estadual de Campinas (UNICAMP) - Laboratory of Emerging Viruses ( email )

Campinas
Brazil

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