See all articles by William M. de SouzaVirology Research Centre, Ribeirão Preto Medical School, University of São Paulo
Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas
National Biosciences Laboratory, Brazilian Centre for Research in Energy and Materials (CNPEM)
National Biosciences Laboratory, Brazilian Centre for Research in Energy and Materials (CNPEM)
Department of Genetics, Evolution, Microbiology and Immunology
Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas
Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas
Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas
Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas
Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas
Autoimmune Research Laboratory, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas
Tropical Medicine Institute, Medical School, University of São Paulo
Hematology and Transfusion Medicine Centre, University of Campinas
Tropical Medicine Institute, Medical School, University of São Paulo
Hematology and Transfusion Medicine Centre, University of Campinas
Hematology and Transfusion Medicine Centre, University of Campinas
Autoimmune Research Laboratory, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas
Brazilian Laboratory on Silencing Technologies, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas
Tropical Medicine Institute, Medical School, University of São Paulo
Hematology and Transfusion Medicine Centre, University of Campinas
Tropical Medicine Institute, Medical School, University of São Paulo
University of Oxford
University of Oxford
Tropical Medicine Institute, Medical School, University of São Paulo
Tropical Medicine Institute, Medical School, University of São Paulo
Tropical Medicine Institute, Medical School, University of São Paulo
Tropical Medicine Institute, Medical School, University of São Paulo
Tropical Medicine Institute, Medical School, University of São Paulo
Tropical Medicine Institute, Medical School, University of São Paulo
Tropical Medicine Institute, Medical School, University of São Paulo
University of São Paulo (USP) - Department of Epidemiology
Hemoam - Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas
Brazilian Laboratory on Silencing Technologies, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas
Tropical Medicine Institute, Medical School, University of São Paulo
Department of Clinical Pathology, School of Medical Sciences, University of Campinas
CDL Laboratório Santos e Vidal Ltda.
Department of Internal Medicine, School of Medical Sciences, University of Campinas
Department of Internal Medicine, School of Medical Sciences, University of Campinas
One Health Laboratory, Feevale University
Animal Virology Laboratory, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas,
Hematology and Transfusion Medicine Centre, University of Campinas
Hematology and Transfusion Medicine Centre, University of Campinas
Universidade Estadual de Campinas (UNICAMP) - Department of Biochemistry and Tissue Biology
DB Diagnósticos do Brasil
Department of Zoology, University of Oxford
University of Southampton; Southampton Statistical Sciences Research Institute
Brazilian Laboratory on Silencing Technologies, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas
Brazilian Center for Research in Energy and Materials (CNPEM) - Brazilian Biosciences National Laboratory (LNBio)
Autoimmune Research Laboratory, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas
Washington University School of Medicine - Department of Medicine
Imperial College London - MRC Centre for Global Infectious Disease Analysis
University of São Paulo (USP) - LIM-46 e LIM-49 do Instituto de Medicina Tropical
Universidade Estadual de Campinas (UNICAMP) - Laboratory of Emerging Viruses
Universidade Estadual de Campinas (UNICAMP) - Laboratory of Emerging Viruses
Abstract
Background: A new SARS-CoV-2 lineage, named P.1 (20J/501Y.V3), has recently been detected in Brazil. Mutations accrued by the P.1 lineage include amino acid changes in the receptor-binding domain of the spike protein that also are reported in variants of concern in the United Kingdom (B.1.1.7) and South Africa (B.1.325).
Methods: We isolated two P.1-containing specimens from nasopharyngeal and bronchoalveolar lavage samples of patients of Manaus, Brazil. We measured neutralization of the P.1 virus after incubation with the plasma of 19 COVID-19 convalescent blood donors and recipients of the chemically-inactivated CoronaVac vaccine and compared these results to neutralization of a SARS-CoV-2 B-lineage previously circulating in Brazil.
Findings: The immune plasma of COVID-19 convalescent blood donors had 6-fold less neutralizing capacity against the P.1 than against the B-lineage. Moreover, five months after booster immunization with CoronaVac, plasma from vaccinated individuals failed to efficiently neutralize P.1 lineage isolates.
Interpretation: These data indicate that the P.1 lineage may escape from neutralizing antibodies generated in response to polyclonal stimulation against previously circulating variants of SARS-CoV-2.
Funding: São Paulo Research Foundation, MCTI/FINEP, Medical Research Council, National Council for Scientific and Technological Development, National Institutes of Health.
Conflict of Interest: M.S.D. is a consultant for Inbios, Vir Biotechnology, NGMBiopharmaceuticals, and Carnival Corporation, and on the Scientific Advisory Boards of Moderna and Immunome. The Diamond laboratory has received funding support in sponsored research agreements from Moderna, Vir Biotechnology, and Emergent BioSolutions.
Ethical Approval: All procedures followed the ethical standards of the responsible committee on humanexperimentation and approved by the ethics committees from the University of Campinas, Brazil (Approval number CONEP 4.021.484 for plasma collection of blood donors, CAEE32078620.4.0000.5404 and 30227920.9.0000.5404 for the sampling of vaccinated and viral genome sequencing, respectively). All patient data were anonymized before study inclusion.Informed consent was obtained from all subjects for being included in the study.
de Souza, William M. and Amorim, Mariene R. and Sesti-Costa, Renata and Coimbra, Lais D. and Toledo-Teixeira, Daniel Augusto de and Parise, Pierina L. and Barbosa, Priscilla P. and Bispo-dos-Santos, Karina and Mofatto, Luciana S. and Simeoni, Camila L. and Brunetti, Natalia S. and Claro, Ingra M. and Duarte, Adriana S. S. and Coletti, Thais M. and Zangirolami, Audrey B. and Costa-Lima, Carolina and Gomes, Arilson Bernardo S. P. and Buscaratti, Lucas I. and Sales, Flavia C. and Costa, Vitor A. and Franco, Lucas A.M. and Candido, Darlan S. and Pybus, Oliver G. and de Jesus, Jaqueline G. and Silva, Camila A. M. and Ramundo, Mariana S. and Ferreira, Giulia M. and Pinho, Mariana C. and Souza, Leandro M. and Rocha, Esmenia C. and Andrade, Pamela S. and Crispim, Myuki A.E. and Maktura, Grazielle C. and Manuli, Erika R. and Santos, Magnun N.N. and Camilo, Cecilia C. and Angerami, Rodrigo N. and Moretti, Maria L. and Spilki, Fernando R. and Arns, Clarice W. and Addas-Carvalho, Marcelo and Benites, Bruno D. and Mori, Marcelo A. and Gaburo, Nelson and Dye, Christopher and Wu, Chieh-Hsi and Marques-Souza, Henrique and Marques, Rafael E. and Farias, Alessandro and Diamond, Michael S. and Faria, Nuno R. and Sabino, Ester Cerdeira and Granja, Fabiana and Proenca-Modena, Jose Luiz, Levels of SARS-CoV-2 Lineage P.1 Neutralization by Antibodies Elicited after Natural Infection and Vaccination. Available at SSRN:
https://ssrn.com/abstract=3793486 or
http://dx.doi.org/10.2139/ssrn.3793486
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