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Single Vaccination with BNT162b2 or ChAdOx1 in Older People Induces Equivalent Antibody Generation but Enhanced Cellular Responses after ChAdOx1

15 Pages Posted: 13 Apr 2021

See all articles by Helen Marie Parry

Helen Marie Parry

University of Birmingham - Institute of Immunology and Immunotherapy

Rachel Bruton

University of Birmingham - Institute of Immunology and Immunotherapy

Gokhan Tut

University of Birmingham - Institute of Immunology and Immunotherapy

Myah Ali

University of Birmingham - Institute of Immunology and Immunotherapy

C Stephens

University of Birmingham - Institute of Immunology and Immunotherapy

Sian Faustini

University of Birmingham - Clinical Immunology Service

A Huissoon

University Hospitals Birmingham NHS Foundation Trust

R Meade

Harborne Medical Practice

Kevin Brown

Public Health England

Gayatri Amirthalingam

Public Health England - Immunisation and Countermeasures Division

Bassam Hallis

Public Health England - National Infection Service

Alex G. Richter

University of Birmingham - Clinical Immunology Service

Jianmin Zuo

University of Birmingham - Institute of Immunology and Immunotherapy

More...

Abstract

Background: Extended-interval Covid vaccination regimens are now used widely in order to accelerate population coverage but the relative immunogenicity of different vaccines in older people remains uncertain.

Methods: We recruited 165 participants aged 80+ years who had received a single dose of either BNT162b2 mRNA or ChAdOx1 adenovirus vaccine and studied adaptive immune responses after 5 weeks.

Findings: Antibody responses against spike protein were detectable in 93% and 87% of mRNA or ChAdOx1 recipients respectively with median antibody titres of 19.3 and 19.6 U/ml (p=0.41). Spike-specific T cell responses were observed in 12% and 31% of mRNA and ChAdOx1 recipients respectively and median responses were 3-fold higher in ChAdOx1 vaccinees at 2 vs 6 spots/million respectively (p=<0.0001). Humoral and cellular immune responses against spike were correlated in both cohorts. Evidence of previous natural infection was seen in 8 donors and associated with 691-fold and 4-fold increase in humoral and cellular immune responses across the whole cohort.

Interpretation: Single doses of either the BNT162b2 or ChAdOx1vaccine in older people thus induce humoral immunity in most donors and are markedly enhanced by previous infection. Cellular responses are weaker but show relative enhancement after the ChAdOx1 platform.

Funding Statement: This work was partially supported by the UK Coronavirus Immunology Consortium (UK-CIC) funded by DHSC/UKRI and the National Core Studies Immunity programme.

Declaration of Interests: The authors declare no conflicts of interest.

Ethics Approval Statement: The work was performed under the CIA UPH IRAS approval (REC 20\NW\0240) and conducted according to the Declaration of Helsinki and good clinical practice.

Keywords: Vaccination, SARS-CoV-2, COVID, Antibody, Cellular, mRNA, ChAdOx1

Suggested Citation

Parry, Helen Marie and Bruton, Rachel and Tut, Gokhan and Ali, Myah and Stephens, C and Faustini, Sian and Huissoon, A and Meade, R and Brown, Kevin and Amirthalingam, Gayatri and Hallis, Bassam and Richter, Alex G. and Zuo, Jianmin, Single Vaccination with BNT162b2 or ChAdOx1 in Older People Induces Equivalent Antibody Generation but Enhanced Cellular Responses after ChAdOx1. Available at SSRN: https://ssrn.com/abstract=3825573 or http://dx.doi.org/10.2139/ssrn.3825573

Helen Marie Parry (Contact Author)

University of Birmingham - Institute of Immunology and Immunotherapy ( email )

Birmingham
United Kingdom

Rachel Bruton

University of Birmingham - Institute of Immunology and Immunotherapy ( email )

Birmingham
United Kingdom

Gokhan Tut

University of Birmingham - Institute of Immunology and Immunotherapy

Myah Ali

University of Birmingham - Institute of Immunology and Immunotherapy ( email )

Birmingham
United Kingdom

C Stephens

University of Birmingham - Institute of Immunology and Immunotherapy ( email )

Birmingham
United Kingdom

Sian Faustini

University of Birmingham - Clinical Immunology Service ( email )

United Kingdom

A Huissoon

University Hospitals Birmingham NHS Foundation Trust

Mindelsohn Way
Edgbaston
Birmingham, B15 2TH
United Kingdom

R Meade

Harborne Medical Practice ( email )

Harborne
United Kingdom

Kevin Brown

Public Health England ( email )

Wellington House,
133-155 Waterloo Rd,
London, SE1 8UG

Gayatri Amirthalingam

Public Health England - Immunisation and Countermeasures Division ( email )

Wellington House
133-155 Waterloo Road
London, SE1 8UG
United Kingdom

Bassam Hallis

Public Health England - National Infection Service ( email )

United Kingdom

Alex G. Richter

University of Birmingham - Clinical Immunology Service ( email )

United Kingdom

Jianmin Zuo

University of Birmingham - Institute of Immunology and Immunotherapy ( email )

Birmingham
United Kingdom

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