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Cellular Bases of Olfactory Circuit Assembly Revealed by Systematic Time-Lapse Imaging

50 Pages Posted: 4 May 2021 Publication Status: Review Complete

See all articles by Tongchao Li

Tongchao Li

Stanford University - Department of Biology

Tian-Ming Fu

Howard Hughes Medical Institute - Janelia Research Campus

Hongjie Li

Stanford University - Department of Biology; Stanford University - Howard Hughes Medical Institute

Qijing Xie

Stanford University - Department of Biology; Stanford University - Neurosciences Graduate Program

David J. Luginbuhl

Stanford University - Department of Biology

Eric Betzig

Howard Hughes Medical Institute - Janelia Research Campus

Liqun Luo

Stanford University - Department of Biology; Stanford University - Department of Bioengineering; Stanford University - Department of Applied Physics; Stanford University - Chan Zuckerberg Biohub

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Abstract

Neural circuit assembly features simultaneous targeting of numerous neuronal processes from constituent neuron types, yet the dynamics is poorly understood. Here, we use the Drosophila olfactory circuit to investigate dynamic cellular processes by which olfactory receptor neurons (ORNs) target axons precisely to specific glomeruli in the ipsi- and contralateral antennal lobes. Time-lapse imaging of individual axons from 28 ORN types revealed a rich diversity in extension speed, innervation timing, and ipsilateral branch locations, and identified that ipsilateral targeting occurs via stabilization of transient interstitial branches. Fast imaging using adaptive optics-corrected lattice light-sheet microscopy showed that upon approaching target, many ORN types exhibit “exploring branches” consisted of parallel microtubule-based terminal branches emanating from an F-actin-rich hub. Antennal nerve ablations uncovered essential roles for bilateral interactions in contralateral target selection, and for ORN axons to facilitate dendritic refinement of postsynaptic partner neurons. Altogether, these observations provide cellular bases for wiring specificity establishment.

Suggested Citation

Li, Tongchao and Fu, Tian-Ming and Li, Hongjie and Xie, Qijing and Luginbuhl, David J. and Betzig, Eric and Luo, Liqun, Cellular Bases of Olfactory Circuit Assembly Revealed by Systematic Time-Lapse Imaging. Available at SSRN: https://ssrn.com/abstract=3839760 or http://dx.doi.org/10.2139/ssrn.3839760
This version of the paper has not been formally peer reviewed.

Tongchao Li

Stanford University - Department of Biology ( email )

Stanford, CA
United States

Tian-Ming Fu

Howard Hughes Medical Institute - Janelia Research Campus ( email )

19700 Helix Drive
Ashburn, VA 20147
United States

Hongjie Li

Stanford University - Department of Biology

Gilbert Building, Rm 109
371 Serra Mall
Stanford, CA 94305
United States

Stanford University - Howard Hughes Medical Institute

Stanford, CA 94305
United States

Qijing Xie

Stanford University - Department of Biology

Gilbert Building, Rm 109
371 Serra Mall
Stanford, CA 94305
United States

Stanford University - Neurosciences Graduate Program

Stanford, CA 94305
United States

David J. Luginbuhl

Stanford University - Department of Biology ( email )

Gilbert Building, Rm 109
371 Serra Mall
Stanford, CA 94305
United States

Eric Betzig

Howard Hughes Medical Institute - Janelia Research Campus ( email )

19700 Helix Drive
Ashburn, VA 20147
United States

Liqun Luo (Contact Author)

Stanford University - Department of Biology ( email )

Stanford, CA
United States

Stanford University - Department of Bioengineering ( email )

Stanford, CA 94305
United States

Stanford University - Department of Applied Physics ( email )

CA
United States

Stanford University - Chan Zuckerberg Biohub

499 Illinois Street
San Francisco, CA 94158
United States

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