Three Stability Indicating Spectrophotometric and Spectrodensitometric Methods for the Determination of Thiocolchicoside in Presence of Its Degradant.
Analytical Chemistry, 2021
15 Pages Posted: 26 May 2021 Last revised: 8 Jun 2021
Date Written: May 25, 2021
Three simple, selective, accurate and green methods were developed for the determination of thiocolchicoside in the presence of its degradation product without preliminary separation or need to an advanced HPLC system. The first derivative spectrophotometry method D1 was developed with good selectivity without interference of the degradation product. The peak amplitudes of D1 spectra of TCC at 412.8 nm (corresponding to the zero-crossing point of degradation product) TCC could be determined over a concentration range of 5-30 μg/ml with mean percentage recovery of 100.28±0.138%. The second method is Dual wave length spectrophotometry DW, Linear correlations were obtained between the differences in absorbance values at 256.8-290.8 nm and the corresponding concentrations of TCC while the degradant shows the same absorbance value. TCC could be determined over a concentration range of 5-40 μg/ml with mean percentage recovery 100.44±0.136%. The third method is TLC densitometric method, It was used for the quantitative determination of thiocolchicoside in presence of its degradation product using silica gel plates and a mixture of ethyl acetate: methanol (85:15 v/v) as a developing system at 254 nm in the range of 4-40 μg/spot with recovery percentage 100.00±1.181%. These methods were checked by the analysis of laboratory-prepared mixtures of TCC and its degradation product in different ratios and its pharmaceutical dosage form without any interference. There was no statistically significant difference in results compared with the official method.
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