puc-header

The Nasal Mutualist Dolosigranulum pigrum AMBR11 Supports Homeostasis via Multiple Mechanisms

40 Pages Posted: 27 May 2021 Publication Status: Review Complete

See all articles by llke De Boeck

llke De Boeck

University of Antwerp

Stijn Wittouck

University of Antwerp - Department of Bioscience Engineering

Katleen Martens

KU Leuven - Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immunology Research Unit

Irina Spacova

University of Antwerp - Department of Bioscience Engineering

Eline Cauwenberghs

University of Antwerp - Department of Bioscience Engineering

Camille Nina Allonsius

University of Antwerp

Jennifer Jörissen

University of Antwerp

Sander Wuyts

University of Antwerp - Department of Bioscience Engineering

Wannes Van Beeck

University of Antwerp

Jelle Dillen

University of Antwerp

Peter A. Bron

University of Antwerp

brecht steelant

KU Leuven - Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immunology Research Unit

Peter W. Hellings

KU Leuven

Olivier M. Vanderveken

Antwerp University Hospital - Multidisciplinary Sleep Disorders Centre

Sarah Lebeer

University of Antwerp - Department of Bioscience Engineering

More...

Abstract

Comparing the nasal microbiome of healthy individuals and chronic rhinosinusitis (CRS) patients revealed Dolosigranulum pigrum as a species clearly associated with nasal health, although it was not yet cultured from healthy individuals. In this study, we explored the properties of this understudied lactic acid bacterium by integrating  comparative genomics, habitat mining, cultivation, and functional characterization of interaction capacities. Mining 10.000 samples from the Earth Microbiome Project of 17 habitat types revealed that Dolosigranulum is mainly associated with the human nasal cavity. D. pigrum AMBR11 isolated from the nose of a healthy individual exerted antimicrobial activity against Staphylococcus aureus , and decreased proinflammatory cytokine production in airway epithelial cells and Galleria mellonella larvae mortality induced by this important nasal pathobiont. Furthermore, the strain protected the nasal barrier function in a mouse model using interleukin-4 as disruptive cytokine. Hence, D. pigrum AMBR11 is a mutualist with high potential as topical live-biotherapeutic product.

Suggested Citation

De Boeck, llke and Wittouck, Stijn and Martens, Katleen and Spacova, Irina and Cauwenberghs, Eline and Allonsius, Camille Nina and Jörissen, Jennifer and Wuyts, Sander and Van Beeck, Wannes and Dillen, Jelle and Bron, Peter A. and steelant, brecht and Hellings, Peter W. and Vanderveken, Olivier M. and Lebeer, Sarah, The Nasal Mutualist Dolosigranulum pigrum AMBR11 Supports Homeostasis via Multiple Mechanisms. Available at SSRN: https://ssrn.com/abstract=3854501 or http://dx.doi.org/10.2139/ssrn.3854501
This version of the paper has not been formally peer reviewed.

Llke De Boeck

University of Antwerp

Stijn Wittouck

University of Antwerp - Department of Bioscience Engineering

Belgium

Katleen Martens

KU Leuven - Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immunology Research Unit

Leuven
Belgium

Irina Spacova

University of Antwerp - Department of Bioscience Engineering

Belgium

Eline Cauwenberghs

University of Antwerp - Department of Bioscience Engineering

Belgium

Camille Nina Allonsius

University of Antwerp

Prinsstraat 13
Antwerp, 2000
Belgium

Jennifer Jörissen

University of Antwerp

Sander Wuyts

University of Antwerp - Department of Bioscience Engineering

Belgium

Wannes Van Beeck

University of Antwerp

Prinsstraat 13
Antwerp, 2000
Belgium

Jelle Dillen

University of Antwerp

Prinsstraat 13
Antwerp, 2000
Belgium

Peter A. Bron

University of Antwerp

Prinsstraat 13
Antwerp, 2000
Belgium

Brecht Steelant

KU Leuven - Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immunology Research Unit ( email )

Leuven
Belgium

Peter W. Hellings

KU Leuven

Oude Markt 13
Leuven, 3000
Belgium

Olivier M. Vanderveken

Antwerp University Hospital - Multidisciplinary Sleep Disorders Centre ( email )

Prinsstraat 13
Antwerp, 2000
Belgium

Sarah Lebeer (Contact Author)

University of Antwerp - Department of Bioscience Engineering

Belgium

Click here to go to Cell.com

Paper statistics

Abstract Views
153
Downloads
3
PlumX Metrics