The Nasal Mutualist Dolosigranulum pigrum AMBR11 Supports Homeostasis via Multiple Mechanisms
40 Pages Posted: 27 May 2021 Publication Status: Review CompleteMore...
Comparing the nasal microbiome of healthy individuals and chronic rhinosinusitis (CRS) patients revealed Dolosigranulum pigrum as a species clearly associated with nasal health, although it was not yet cultured from healthy individuals. In this study, we explored the properties of this understudied lactic acid bacterium by integrating comparative genomics, habitat mining, cultivation, and functional characterization of interaction capacities. Mining 10.000 samples from the Earth Microbiome Project of 17 habitat types revealed that Dolosigranulum is mainly associated with the human nasal cavity. D. pigrum AMBR11 isolated from the nose of a healthy individual exerted antimicrobial activity against Staphylococcus aureus , and decreased proinflammatory cytokine production in airway epithelial cells and Galleria mellonella larvae mortality induced by this important nasal pathobiont. Furthermore, the strain protected the nasal barrier function in a mouse model using interleukin-4 as disruptive cytokine. Hence, D. pigrum AMBR11 is a mutualist with high potential as topical live-biotherapeutic product.
Suggested Citation: Suggested Citation