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In-situ Observation of Mitochondrial Biogenesis as the Early Event of Apoptosis

71 Pages Posted: 27 May 2021 Publication Status: Published

See all articles by Chang-Sheng Shao

Chang-Sheng Shao

Chinese Academy of Sciences (CAS) - CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology

Xiu-Hong Zhou

Anhui Agricultural University - Center of Biology

Yu-Hui Miao

University of Science and Technology of China (USTC) - The First Affiliated Hospital of USTC, Department of Medical Oncology

Peng Wang

Chinese Academy of Sciences (CAS) - CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology

Qian-Qian Zhang

Chinese Academy of Sciences (CAS) - CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology

Qing Huang

Chinese Academy of Sciences (CAS) - CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology

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Abstract

Mitochondrial biogenesis is a cell response to external stimuli which is generally believed to suppress apoptosis. However, during the process of apoptosis, whether mitochondrial biogenesis occurs in the early stage of the apoptotic cells remains unclear. To address this question, we constructed the COX8-EGFP-ACTIN-mCherry HeLa cells with recombinant fluorescent proteins respectively tagged on the nucleus and mitochondria, and monitored the mitochondrial changes in living cells exposed to gamma-ray radiation. Besides in situ detection of mitochondrial fluorescence changes, we also examined the cell viability, nuclear DNA damage, reactive oxygen species (ROS), Mitochondrial superoxide, citrate synthase activity, ATP, cytoplasmic and mitochondrial calcium, mitochondrial DNA copy number and expression of transcription genes related to mitochondrial biogenesis as well as the apoptosis biomarkers. As a result, we confirmed that significant mitochondrial biogenesis took place preceding the radiation-induced apoptosis, and the change of mitochondrial biogenesis at early time was closely correlated with the apoptotic cells at late stage. The involved mechanism was also discussed.

Keywords: mitochondrial biogenesis, apoptosis, fluorescent proteins, fluorescence

Suggested Citation

Shao, Chang-Sheng and Zhou, Xiu-Hong and Miao, Yu-Hui and Wang, Peng and Zhang, Qian-Qian and Huang, Qing, In-situ Observation of Mitochondrial Biogenesis as the Early Event of Apoptosis. Available at SSRN: https://ssrn.com/abstract=3855073 or http://dx.doi.org/10.2139/ssrn.3855073
This version of the paper has not been formally peer reviewed.

Chang-Sheng Shao

Chinese Academy of Sciences (CAS) - CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology ( email )

Hefei
China

Xiu-Hong Zhou

Anhui Agricultural University - Center of Biology

130 Changjiang W Rd, Shushan Qu
Hefei, 230031
China

Yu-Hui Miao

University of Science and Technology of China (USTC) - The First Affiliated Hospital of USTC, Department of Medical Oncology ( email )

96, Jinzhai Road
Hefei, Anhui 230026
China

Peng Wang

Chinese Academy of Sciences (CAS) - CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology

Hefei
China

Qian-Qian Zhang

Chinese Academy of Sciences (CAS) - CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology

Hefei
China

Qing Huang (Contact Author)

Chinese Academy of Sciences (CAS) - CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology ( email )

Hefei
China

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