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Whole Genome Sequence and Experimental Proteome of Candidatus 'Phormidium Alkaliphilum', an Abundant and Widespread Cyanobacterium With Applications in Biotechnology

39 Pages Posted: 2 Jun 2021 Publication Status: Published

See all articles by Maryam Ataeian

Maryam Ataeian

University of Calgary - Department of Geoscience

Agasteswar Vadlamani

University of Calgary - Department of Geoscience

Marianne Haines

University of Calgary - Department of Geoscience

Damon Mosier

University of Calgary - Department of Geoscience

Xiaoli Dong

University of Calgary - Department of Geoscience

Manuel Kleiner

North Carolina State University - Department of Plant and Microbial Biology

Marc Strous

University of Calgary - Department of Geoscience

Alyse Hawley

University of Calgary - Department of Geoscience

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Abstract

Cyanobacteria encompass a diverse group of photoautotrophic bacteria with important roles in nature and biotechnology. Here we introduce Candidatus “Phormidium alkaliphilum”, an abundant member of alkaline soda lake microbial communities across the globe. Multiple, independent studies have reported successful colonization of photobioreactors for biotechnology applications by Ca. “P. alkaliphilum” and closely related cyanobacteria. The complete, circular whole genome sequence of Ca. “P. alkaliphilum” was obtained using combined Nanopore and Illumina sequencing of a consortium abundant in Ca. “P. alkaliphilum”. Strain heterogeneity of the Ca. “P. alkaliphilum” population was shown to contribute to photobioreactor robustness under different operational conditions.  Comparative genomics of closely related Phormidium species showed that adaption to high pH and alkalinity could not be attributed to specific genes. Proteomics showed high pH (11.2) provoked only minimal changes in gene expression in Ca. “P. alkaliphilum”, compared to control cultures at pH 9. Diversity of photosystem antennae proteins, a high affinity terminal oxidase and ecophysiological flexibility contributed to success of Ca. “P. alkaliphilum” in photobioreactors and biotechnology, compared to other soda lake cyanobacteria.

Suggested Citation

Ataeian, Maryam and Vadlamani, Agasteswar and Haines, Marianne and Mosier, Damon and Dong, Xiaoli and Kleiner, Manuel and Strous, Marc and Hawley, Alyse, Whole Genome Sequence and Experimental Proteome of Candidatus 'Phormidium Alkaliphilum', an Abundant and Widespread Cyanobacterium With Applications in Biotechnology. Available at SSRN: https://ssrn.com/abstract=3858915 or http://dx.doi.org/10.2139/ssrn.3858915
This version of the paper has not been formally peer reviewed.

Maryam Ataeian

University of Calgary - Department of Geoscience ( email )

University Drive
Calgary, T2N 1N4
Canada

Agasteswar Vadlamani

University of Calgary - Department of Geoscience ( email )

Earth Science 118
2500 University Dr. NW
Calgary, Alberta T2N 1N4
Canada

Marianne Haines

University of Calgary - Department of Geoscience ( email )

Earth Science 118
2500 University Dr. NW
Calgary, Alberta T2N 1N4
Canada

Damon Mosier

University of Calgary - Department of Geoscience ( email )

University Drive
Calgary, T2N 1N4
Canada

Xiaoli Dong

University of Calgary - Department of Geoscience ( email )

University Drive
Calgary, T2N 1N4
Canada

Manuel Kleiner

North Carolina State University - Department of Plant and Microbial Biology ( email )

Hillsborough Street
Raleigh, NC 27695
United States

Marc Strous

University of Calgary - Department of Geoscience ( email )

Earth Science 118
2500 University Dr. NW
Calgary, Alberta T2N 1N4
Canada

Alyse Hawley (Contact Author)

University of Calgary - Department of Geoscience ( email )

Earth Science 118
2500 University Dr. NW
Calgary, Alberta T2N 1N4
Canada

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