UHPLC Coupled with Charged Aerosol Detector for Rapid Separation of Steviol Glycosides in Commercial Sweeteners and Extract of Stevia Rebaudiana

24 Pages Posted: 28 Jun 2021

See all articles by Marcela Hollá

Marcela Hollá

Charles University in Prague

Dalibor Šatínský

Charles University in Prague

František Švec

Charles University in Prague

Hana Sklenarova

Charles University in Prague

Date Written: 2021

Abstract

Natural sweeteners are in high demand as a part of a healthy lifestyle. Among them, sweeteners with decreased caloric value and suitability for diabetes patients are most requested. Extension in their consumption extends the need for their quality control. A fast gradient UHPLC coupled with charged aerosol detection enabling quantitation of stevioside, rebaudioside A-D, and steviolbioside in commercial sweeteners and Stevia rebaudiana plant extracts has been developed. The method was developed to achieve high efficiency, simplicity, versatility, and low solvent consumption. All steviol glycosides were baseline-separated in less than 4 min with a total run time of 7 min. Buffer-free eluents were used in the separations and only 2.45 mL solvent were needed per analysis. The state of the art column Luna Omega Polar featuring polar modification of C18 stationary phase was used for the excellent separation of polar steviol glycosides. Critical pair of glycosides, stevioside and rebaudioside A, were baseline separated with a resolution of 2.41. The universal charged aerosol detector allowed quantitation of steviol glycosides with a limit of detection and quantitation 0.15 and 0.5 µg/mL, respectively. Method intra-day precision was less than 2% (RSD), and the recovery was 89.6-105.0% and 93.8-111.4% for plant material and sweetener tablets, respectively. The quantity of steviol glycosides in three out of four commercial sweeteners was 3.0-12.3% higher than declared. The content was about 12.4% less than declared in one sample. The most abundant glycoside detected in sweeteners was stevioside followed by rebaudioside A. A leaf-to-stem ratio describing the dominant accumulation of steviol glycosides in leaves affected the differences in the amount of steviol glycosides among plant samples.

Suggested Citation

Hollá, Marcela and Šatínský, Dalibor and Švec, František and Sklenarova, Hana, UHPLC Coupled with Charged Aerosol Detector for Rapid Separation of Steviol Glycosides in Commercial Sweeteners and Extract of Stevia Rebaudiana (2021). Available at SSRN: https://ssrn.com/abstract=3875187 or http://dx.doi.org/10.2139/ssrn.3875187

Marcela Hollá

Charles University in Prague ( email )

Celetná 13
Praha 1, 116 36
Czech Republic

Dalibor Šatínský

Charles University in Prague ( email )

Celetná 13
Praha 1, 116 36
Czech Republic

František Švec

Charles University in Prague ( email )

Celetná 13
Praha 1, 116 36
Czech Republic

Hana Sklenarova (Contact Author)

Charles University in Prague ( email )

Celetná 13
Praha 1, 116 36
Czech Republic

Do you have a job opening that you would like to promote on SSRN?

Paper statistics

Downloads
12
Abstract Views
82
PlumX Metrics