puc-header

Ftsz-Mediated Fission of a Cuboid Bacterial Symbiont

25 Pages Posted: 13 Jul 2021 Publication Status: Published

See all articles by Philipp Weber

Philipp Weber

University of Vienna - Department of Ecogenomics and Systems Biology

Gabriela Fabiola Paredes

University of Vienna - Department of Ecogenomics and Systems Biology

Tobias Viehboeck

University of Vienna - Department of Ecogenomics and Systems Biology

Nika Pende

University of Paris-Saclay - Institut Pasteur; University of Vienna - Department of Ecogenomics and Systems Biology

Jean-Marie Volland

University of Vienna - Department of Ecogenomics and Systems Biology

Olivier Gros

Université des Antilles - C3MAG, UFR Des Sciences Exactes Et Naturelles

Joerg Ott

University of Vienna - Department of Limnology and Bio-Oceanography

Silvia Bulgheresi

University of Vienna - Department of Ecogenomics and Systems Biology

More...

Abstract

Less than a handful of cuboid and squared cells have been described in nature which makes them a rarity. Here, we show how  Candidatus Thiosymbion cuboideus, a cube-like gammaproteobacterium, reproduces on the surface of marine free-living nematodes. Morphometric analysis showed that most non-dividing  Ca . T. cuboideus cells are squared and 1.3 μm-thick on average. Immunostaining of symbiont cells with an anti-fimbriae antibody revealed that they are host-polarized, as these appendages exclusively localized at the host-proximal (animal-attached) pole. Moreover, by applying a fluorescently labelled metabolic probe to track new cell wall insertion  in vivo , we observed that the host-attached pole started to septate before the distal one. Proximal-to-distal insertion of new cell wall material at the septum displayed a similar localization pattern as the tubulin homolog FtsZ. Although this has been shown to arrange into squares in synthetically remodelled cuboid cells, here we show that FtsZ may also mediate the division of naturally occurring ones. This implies that, even in natural settings, membrane roundness is not required for FtsZ function.

Suggested Citation

Weber, Philipp and Paredes, Gabriela Fabiola and Viehboeck, Tobias and Pende, Nika and Pende, Nika and Volland, Jean-Marie and Gros, Olivier and Ott, Joerg and Bulgheresi, Silvia, Ftsz-Mediated Fission of a Cuboid Bacterial Symbiont. Available at SSRN: https://ssrn.com/abstract=3885987 or http://dx.doi.org/10.2139/ssrn.3885987
This version of the paper has not been formally peer reviewed.

Philipp Weber

University of Vienna - Department of Ecogenomics and Systems Biology ( email )

Austria

Gabriela Fabiola Paredes

University of Vienna - Department of Ecogenomics and Systems Biology ( email )

Austria

Tobias Viehboeck

University of Vienna - Department of Ecogenomics and Systems Biology ( email )

Austria

Nika Pende

University of Paris-Saclay - Institut Pasteur ( email )

75724 Paris CEDEX 15
France

University of Vienna - Department of Ecogenomics and Systems Biology ( email )

Austria

Jean-Marie Volland

University of Vienna - Department of Ecogenomics and Systems Biology ( email )

Austria

Olivier Gros

Université des Antilles - C3MAG, UFR Des Sciences Exactes Et Naturelles ( email )

ESPE - Université des Antilles
Route du phare - Pointe des nègres - BP 678
Fort de France, Martinique 97262
France

Joerg Ott

University of Vienna - Department of Limnology and Bio-Oceanography ( email )

Bruenner Strasse 72
Vienna, Vienna 1090
Austria

Silvia Bulgheresi (Contact Author)

University of Vienna - Department of Ecogenomics and Systems Biology ( email )

Austria

Click here to go to Cell.com

Paper statistics

Abstract Views
268
Downloads
9
PlumX Metrics