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Long-Term Analysis of Antibodies Elicited by Sputnik V in Tucuman, Argentina

20 Pages Posted: 12 Aug 2021

See all articles by Rossana Elena Chahla

Rossana Elena Chahla

Tucumán Public Healthcare Administration (SIPROSA)

Rodrigo Hernán Tomás Grau

National University of Tucuman - Instituto de Medicina Molecular y Celular Aplicada - IMMCA (UNT-CONICET-SIPROSA)

Silvia Inés Cazorla

CONICET - Centro de Referencia para Lactobacilos – CERELA

Diego Ploper

National University of Tucuman - Instituto de Medicina Molecular y Celular Aplicada - IMMCA (UNT-CONICET-SIPROSA)

Esteban Vera Pingitore

National University of Tucuman - Instituto de Medicina Molecular y Celular Aplicada - IMMCA (UNT-CONICET-SIPROSA)

Mónica Aguilar López

Néstor Kirchner Hospital - Central Public Health laboratory (LSP) (SIPROSA)

Patricia Aznar

Néstor Kirchner Hospital - Central Public Health laboratory (LSP) (SIPROSA)

María Elena Alcorta

Néstor Kirchner Hospital - Central Public Health laboratory (LSP) (SIPROSA)

Eva María del Mar Vélez

Néstor Kirchner Hospital - Central Public Health laboratory (LSP) (SIPROSA)

Aguístn Stagnetto

National University of Tucuman - Instituto de Medicina Molecular y Celular Aplicada - IMMCA (UNT-CONICET-SIPROSA)

César Luis Avila

National University of Tucuman - Instituto de Medicina Molecular y Celular Aplicada - IMMCA (UNT-CONICET-SIPROSA)

Carolina Maldonado Galdeano

CONICET - Centro de Referencia para Lactobacilos – CERELA

Sergio Benjamín Socías

National University of Tucuman - Instituto de Medicina Molecular y Celular Aplicada - IMMCA (UNT-CONICET-SIPROSA)

Dar Heinze

Boston University - Center for Regenerative Medicine

Silvia Adriana Navarro

National University of Tucuman - Instituto de Medicina Molecular y Celular Aplicada - IMMCA (UNT-CONICET-SIPROSA)

Conrado Jose LLapur

Tucumán Public Healthcare Administration (SIPROSA)

Dardo Costa

Néstor Kirchner Hospital - Central Public Health laboratory (LSP) (SIPROSA)

Isolina Flores

Néstor Kirchner Hospital - Central Public Health laboratory (LSP) (SIPROSA)

Alexis Edelstein

Argentina Ministry of Health-Ministerio de Salud Argentina - National Administration of Laboratories and Health Institutes of Argentina (ANLIS), Dr. Carlos G. Malbrán

Shreyas Kowdle

Mount Sinai School of Medicine - Department of Microbiology

Claudia Perandones

Argentina Ministry of Health-Ministerio de Salud Argentina - National Administration of Laboratories and Health Institutes of Argentina (ANLIS), Dr. Carlos G. Malbrán

Benhur Lee

Mount Sinai School of Medicine - Department of Microbiology

Gabriela Apfelbaum

Universidad Nacional de Tucumán - School of Medicine

Raul Mostoslavsky

Harvard Medical School - Cancer Center

Gustavo Mostoslavsky

Boston University - Center for Regenerative Medicine

Gabriela Perdigón

CONICET - Centro de Referencia para Lactobacilos – CERELA

Rosana Chehin

National University of Tucuman - Instituto de Medicina Molecular y Celular Aplicada - IMMCA (UNT-CONICET-SIPROSA)

More...

Abstract

Background: Gam-COVID-Vac, also known as SPUTNIK V, is the first COVID-19 vaccine registered, has emergency use authorization in 70 nations, and has been administered to millions worldwide. However, there are very few peer-reviewed studies describing its effects. As independent reports begin to be published, a more accurate picture regarding safety and effectiveness could accelerate approval by the WHO.

Methods: An ELISA that detects anti-SARS-CoV-2-spike-RBD IgG was used, in both transversal and longitudinal studies, to analyze humoral immune responses in 602 healthcare workers who received SPUTNIK V between December 2020 and July 2021.Findings: Seroconversion was detected in 97% of individuals after 28 days post-vaccination (dpv). Anti-RBD titers began to decrease after 60 dpv, but remained detectable in 94% of volunteers at 90 dpv. At 180 dpv, anti-RDB titers persisted in 31% of volunteers. Previous SARS-CoV-2 infection triggered increased immune response to the first dose, and increased neutralization activity against different variants of concern. The second dose in previously infected individuals further increased anti-RBD titers, even after 90 dpv. However, not all individuals with previous infection responded equally, as only those with basal titers above a certain threshold showed robust responses. Time elapsed between COVID-19 diagnosis and vaccination did not influence titers elicited.

Interpretation: Data presented herein provides essential knowledge regarding the kinetics of antibodies induced by SPUTNIK V up to six months after immunization, and suggests that when considering one-dose vaccination policies for individuals with previous SARS-CoV-2 infection, serological studies to determine basal titers may be important, independent of when diagnosis occurred.

Funding Statement: This research was supported by the Ministry of Public Health of Tucumán (Argentina), Argentinean Research Council-CONICET (PIP 722 and 806), Argentinean Research Agency-MINCYT grants (PICT-2018-3379 and PICT2018-02989), National University of Tucuman (PIUNT-UNT D644/1 and D624), and Florencio Fiorini Foundation.

Declaration of Interests: B. L. is a named inventor on a patent filed by the Icahn School of Medicine which includes the 293T-ACE2-TMPRSS2 (F8-2) cells used for the virus neutralization assay. All other have nothing to declare.

Ethics Approval Statement: Protocols were approved by the regional ethics research boards (Ex. N° 3929-410-P-2020), following the Declaration of Helsinki. Personal data from all volunteers were encrypted. Eligibility criteria were age between 18 to 60 years without any COVID-19 symptoms at the time of vaccination. Individuals provided a signed informed consent to be included in the database of the present study.

Suggested Citation

Chahla, Rossana Elena and Tomás Grau, Rodrigo Hernán and Cazorla, Silvia Inés and Ploper, Diego and Vera Pingitore, Esteban and Aguilar López, Mónica and Aznar, Patricia and Alcorta, María Elena and Vélez, Eva María del Mar and Stagnetto, Aguístn and Avila, César Luis and Maldonado Galdeano, Carolina and Socías, Sergio Benjamín and Heinze, Dar and Navarro, Silvia Adriana and LLapur, Conrado Jose and Costa, Dardo and Flores, Isolina and Edelstein, Alexis and Kowdle, Shreyas and Perandones, Claudia and Lee, Benhur and Apfelbaum, Gabriela and Mostoslavsky, Raul and Mostoslavsky, Gustavo and Perdigón, Gabriela and Chehin, Rosana, Long-Term Analysis of Antibodies Elicited by Sputnik V in Tucuman, Argentina. Available at SSRN: https://ssrn.com/abstract=3902468 or http://dx.doi.org/10.2139/ssrn.3902468

Rossana Elena Chahla

Tucumán Public Healthcare Administration (SIPROSA) ( email )

Tucumán
Argentina

Rodrigo Hernán Tomás Grau

National University of Tucuman - Instituto de Medicina Molecular y Celular Aplicada - IMMCA (UNT-CONICET-SIPROSA) ( email )

Tucumán
Argentina

Silvia Inés Cazorla

CONICET - Centro de Referencia para Lactobacilos – CERELA ( email )

Tucumán
Argentina

Diego Ploper

National University of Tucuman - Instituto de Medicina Molecular y Celular Aplicada - IMMCA (UNT-CONICET-SIPROSA) ( email )

Tucumán
Argentina

Esteban Vera Pingitore

National University of Tucuman - Instituto de Medicina Molecular y Celular Aplicada - IMMCA (UNT-CONICET-SIPROSA) ( email )

Tucumán
Argentina

Mónica Aguilar López

Néstor Kirchner Hospital - Central Public Health laboratory (LSP) (SIPROSA) ( email )

Tucumán
Argentina

Patricia Aznar

Néstor Kirchner Hospital - Central Public Health laboratory (LSP) (SIPROSA) ( email )

Tucumán
Argentina

María Elena Alcorta

Néstor Kirchner Hospital - Central Public Health laboratory (LSP) (SIPROSA) ( email )

Tucumán
Argentina

Eva María del Mar Vélez

Néstor Kirchner Hospital - Central Public Health laboratory (LSP) (SIPROSA) ( email )

Tucumán
Argentina

Aguístn Stagnetto

National University of Tucuman - Instituto de Medicina Molecular y Celular Aplicada - IMMCA (UNT-CONICET-SIPROSA) ( email )

Tucumán
Argentina

César Luis Avila

National University of Tucuman - Instituto de Medicina Molecular y Celular Aplicada - IMMCA (UNT-CONICET-SIPROSA) ( email )

Tucumán
Argentina

Carolina Maldonado Galdeano

CONICET - Centro de Referencia para Lactobacilos – CERELA ( email )

Tucumán
Argentina

Sergio Benjamín Socías

National University of Tucuman - Instituto de Medicina Molecular y Celular Aplicada - IMMCA (UNT-CONICET-SIPROSA) ( email )

Tucumán
Argentina

Dar Heinze

Boston University - Center for Regenerative Medicine ( email )

United States

Silvia Adriana Navarro

National University of Tucuman - Instituto de Medicina Molecular y Celular Aplicada - IMMCA (UNT-CONICET-SIPROSA) ( email )

Tucumán
Argentina

Conrado Jose LLapur

Tucumán Public Healthcare Administration (SIPROSA) ( email )

Tucumán
Argentina

Dardo Costa

Néstor Kirchner Hospital - Central Public Health laboratory (LSP) (SIPROSA) ( email )

Tucumán
Argentina

Isolina Flores

Néstor Kirchner Hospital - Central Public Health laboratory (LSP) (SIPROSA) ( email )

Tucumán
Argentina

Alexis Edelstein

Argentina Ministry of Health-Ministerio de Salud Argentina - National Administration of Laboratories and Health Institutes of Argentina (ANLIS), Dr. Carlos G. Malbrán ( email )

Buenos Aires
Argentina

Shreyas Kowdle

Mount Sinai School of Medicine - Department of Microbiology ( email )

New York, NY
United States

Claudia Perandones

Argentina Ministry of Health-Ministerio de Salud Argentina - National Administration of Laboratories and Health Institutes of Argentina (ANLIS), Dr. Carlos G. Malbrán ( email )

Buenos Aires
Argentina

Benhur Lee

Mount Sinai School of Medicine - Department of Microbiology ( email )

New York, NY
United States

Gabriela Apfelbaum

Universidad Nacional de Tucumán - School of Medicine ( email )

Tucumán
Argentina

Raul Mostoslavsky

Harvard Medical School - Cancer Center ( email )

Boston, MA 02114
United States

Gustavo Mostoslavsky

Boston University - Center for Regenerative Medicine ( email )

United States

Gabriela Perdigón

CONICET - Centro de Referencia para Lactobacilos – CERELA ( email )

Tucumán
Argentina

Rosana Chehin (Contact Author)

National University of Tucuman - Instituto de Medicina Molecular y Celular Aplicada - IMMCA (UNT-CONICET-SIPROSA) ( email )

Tucumán
Argentina

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