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Cross-Species Tropism and Antigenic Landscapes of Circulating SARS-CoV-2 Variants

59 Pages Posted: 9 Sep 2021 Publication Status: Under Review

See all articles by Yali Zhang

Yali Zhang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Min Wei

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

yangtao wu

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Juan Wang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Yuting Hong

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Yang Huang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Lunzhi Yuan

Xiamen University

Jian Ma

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Kai Wang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Shaojuan Wang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Yang Shi

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Zikang Wang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Huilin Guo

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Jin Xiao

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Chuanlai Yang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Jianghui Ye

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Jijing Chen

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Yuxi Liu

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Baorong Fu

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Miaolin Lan

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Peixuan Gong

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Zehong Huang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Yingying Su

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Yixin Chen

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Tianying Zhang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Jun Zhang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Huachen Zhu

The University of Hong Kong

Hai Yu

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Quan Yuan

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Tong Cheng

Xiamen University

Yi Guan

The University of Hong Kong

Ningshao Xia

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

More...

Abstract

Mutations in the SARS-CoV-2 spike receptor-binding domain (RBD) may alter the viral host tropism and affect the activities of neutralizing antibodies (nAbs). Here, we investigated 153 RBD mutants and 10 globally circulating VOC/VOI variants for their cross-species infection potentials and sensitivities to nAbs. Several RBD mutations strengthened viral infectivity in cells-expressing ACE2 orthologs of non-human animals, particularly those less susceptible to the ancestral strain. Antigenic profiles revealed the mutations surrounding aa 439-448 and aa 484 were more likely to cause neutralization resistance. Strikingly, the enhanced cross-species infection potentials to the mouse and ferret, instead of the neutralization-escape scores of the mutations, accounted for the positive correlation with the current cumulative prevalence of mutations in humans. Our results provided prospective landscapes for the cross-species transmission potentials and antigenic drift of circulating spike mutants and highlighted the possible spillover of variants to originally-insusceptible animals in associating with their quick spread worldwide.

Funding: This study was supported by the National Natural Science Foundation of China 81902057 (to Y.L.Z.), U1905205 (to Q.Y.), and 81871316 (to Q.Y.) and the CAMS Innovation Fund for Medical Sciences (No. 2019RU022 to N.X.).

Declaration of Interests: None to declare.

Ethics Approval Statement: The study was approved (number: SPHIRB-202101) by the institutional review board of the School of Public Health of Xiamen University following the Declaration of Helsinki, and written informed consent was obtained.Animal studies were carried out in strict accordance with the recommendations of the Guide for the Care and Use of Laboratory
Animals, and were conducted under the approval of the Institutional Animal Care and Use Committee of Xiamen University (mouse experiments) or JOINN Laboratories, Inc. (monkey experiments).

Keywords: SARS-CoV-2 variants, RBD mutations, cross-species tropism, neutralization resistance

Suggested Citation

Zhang, Yali and Wei, Min and wu, yangtao and Wang, Juan and Hong, Yuting and Huang, Yang and Yuan, Lunzhi and Ma, Jian and Wang, Kai and Wang, Shaojuan and Shi, Yang and Wang, Zikang and Guo, Huilin and Xiao, Jin and Yang, Chuanlai and Ye, Jianghui and Chen, Jijing and Liu, Yuxi and Fu, Baorong and Lan, Miaolin and Gong, Peixuan and Huang, Zehong and Su, Yingying and Chen, Yixin and Zhang, Tianying and Zhang, Jun and Zhu, Huachen and Yu, Hai and Yuan, Quan and Cheng, Tong and Guan, Yi and Xia, Ningshao, Cross-Species Tropism and Antigenic Landscapes of Circulating SARS-CoV-2 Variants. Available at SSRN: https://ssrn.com/abstract=3917167 or http://dx.doi.org/10.2139/ssrn.3917167
This version of the paper has not been formally peer reviewed.

Yali Zhang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Xiamen
China

Min Wei

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Xiamen
China

Yangtao Wu

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics ( email )

Xiamen
China

Juan Wang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Xiamen
China

Yuting Hong

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics ( email )

Xiamen
China

Yang Huang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Xiamen
China

Lunzhi Yuan

Xiamen University

Xiamen, 361005
China

Jian Ma

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Xiamen
China

Kai Wang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Xiamen
China

Shaojuan Wang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics ( email )

Xiamen
China

Yang Shi

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Xiamen
China

Zikang Wang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Xiamen
China

Huilin Guo

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics ( email )

Xiamen
China

Jin Xiao

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Xiamen
China

Chuanlai Yang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics ( email )

Xiamen
China

Jianghui Ye

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics ( email )

Xiamen
China

Jijing Chen

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics ( email )

Xiamen
China

Yuxi Liu

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Xiamen
China

Baorong Fu

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics ( email )

Xiamen
China

Miaolin Lan

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics ( email )

Xiamen
China

Peixuan Gong

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics ( email )

Xiamen
China

Zehong Huang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics ( email )

Xiamen
China

Yingying Su

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Xiamen
China

Yixin Chen

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Xiamen
China

Tianying Zhang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Xiamen
China

Jun Zhang

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics ( email )

Xiamen
China

Huachen Zhu

The University of Hong Kong

Pokfulam Road
Hong Kong, HK
China

Hai Yu

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics

Xiamen
China

Quan Yuan

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics ( email )

Xiamen
China

Tong Cheng

Xiamen University

Yi Guan

The University of Hong Kong

Pokfulam Road
Hong Kong, HK
China

Ningshao Xia (Contact Author)

Xiamen University - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics ( email )

Xiamen
China

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