Multifaceted Effects of Catalpol on Promotion of Perforator Flaps Survival in Rats

27 Pages Posted: 5 Oct 2021

See all articles by Yihan Wang

Yihan Wang

Wenzhou Medical University - Department of Orthopaedics

Dingchao Zhu

Wenzhou Medical University - Department of Orthopaedics

Jiajing Xu

Wenzhou Medical University - Department of Orthopaedics

Mingwei Dong

Wenzhou Medical University - Department of Orthopaedics

Zeming Sun

Wenzhou Medical University - Department of Orthopaedics

Zexin Chen

Wenzhou Medical University - Department of Orthopaedics

Yan Lin

Wenzhou Medical University - Department of Orthopaedics

Yao-Sen Wu

Wenzhou Medical University - Department of Orthopaedics

Abstract

Background: Perforator flap replantation is widely used to repair skin defects. However, ischaemia and ischaemia-reperfusion injury lead to flap necrosis, which limits the clinical application of this technique. Catalpol is the main active component of Rehmannia glutinosa. It is an iridoid glucoside compound with a variety of biological activities. We investigated whether Catalpol could promote perforator flap survival.

Methods: Rats were randomly divided into control and Catalpol groups (n = 30 per group). On the 7th day after the operation, flap tissue was obtained to evaluate flap viability, flap perfusion, angiogenesis, apoptosis, oxidative stress and inflammation-related protein levels.

Results: Unlike in the control group, Catalpol promoted the survival of the perforator flap, promoted angiogenesis, reduced apoptosis, alleviated oxidative stress, and depressed expressions of inflammatory proteins.

Conclusions: Catalpol promotes the survival of perforator flaps by promoting angiogenesis, inhibiting apoptosis and oxidative stress, and dampening inflammation.

Note:
Funding Information: This study is supported by Zhejiang Province Public Welfare Technology Application Research Project (Public Welfare Technology Application Research Project of Zhejiang Province) - LGF20H060013 [Wu] and Science and Technology Department of Zhejiang Province - 2020KY190 [Wu].

Declaration of Interests: The authors hereby declare that they have no competing interests.

Ethics Approval Statement: All animal experiments were approved by the Committee for Research on Animals of State Medical University (wydw2017-0022) and were performed in accordance with the Guidelines for the Practice and Use of Animals by the China Health Research Institute.

Keywords: Catalpol, Perforator flap, angiogenesis, APOPTOSIS, Oxidative stress, Inflammation

Suggested Citation

Wang, Yihan and Zhu, Dingchao and Xu, Jiajing and Dong, Mingwei and Sun, Zeming and Chen, Zexin and Lin, Yan and Wu, Yao-Sen, Multifaceted Effects of Catalpol on Promotion of Perforator Flaps Survival in Rats. Available at SSRN: https://ssrn.com/abstract=3932862 or http://dx.doi.org/10.2139/ssrn.3932862

Yihan Wang

Wenzhou Medical University - Department of Orthopaedics

Wenzhou
China

Dingchao Zhu

Wenzhou Medical University - Department of Orthopaedics ( email )

Wenzhou
China

Jiajing Xu

Wenzhou Medical University - Department of Orthopaedics ( email )

Wenzhou
China

Mingwei Dong

Wenzhou Medical University - Department of Orthopaedics

Wenzhou
China

Zeming Sun

Wenzhou Medical University - Department of Orthopaedics ( email )

Wenzhou
China

Zexin Chen

Wenzhou Medical University - Department of Orthopaedics

Wenzhou
China

Yan Lin

Wenzhou Medical University - Department of Orthopaedics ( email )

Wenzhou
China

Yao-Sen Wu (Contact Author)

Wenzhou Medical University - Department of Orthopaedics ( email )

Wenzhou
China

Do you have a job opening that you would like to promote on SSRN?

Paper statistics

Downloads
6
Abstract Views
54
PlumX Metrics