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Gut Ruminococcaceae Levels Correlate with Risk of Antibiotic-Associated Diarrhea

44 Pages Posted: 14 Oct 2021 Publication Status: Published

See all articles by Xiaoqiong Gu

Xiaoqiong Gu

Singapore-MIT Alliance for Research and Technology

Jean XY Sim

National University of Singapore (NUS) - Department of Infection Prevention and Epidemiology

Wei Lin Lee

Singapore-MIT Alliance for Research and Technology - Antimicrobial Resistance Interdisciplinary Research Group

Liang Cui

Singapore-MIT Alliance for Research and Technology

Yvonne FZ Chan

National University of Singapore (NUS) - Department of Infectious Diseases

Ega Danu Chang

Singapore-MIT Alliance for Research and Technology - Antimicrobial Resistance Interdisciplinary Research Group

Yii Ean Teh

National University of Singapore (NUS) - Department of Infectious Diseases

An-Ni Zhang

Massachusetts Institute of Technology (MIT) - Department of Biological Engineering

Federica Armas

Singapore-MIT Alliance for Research and Technology - Antimicrobial Resistance Interdisciplinary Research Group

Franciscus Chandra

Singapore-MIT Alliance for Research and Technology - Antimicrobial Resistance Interdisciplinary Research Group

Chen Hongjie

Singapore-MIT Alliance for Research and Technology - Antimicrobial Resistance Interdisciplinary Research Group

Shijie Zhao

Massachusetts Institute of Technology (MIT) - Department of Biological Engineering

Zhanyi Lee

Singapore-MIT Alliance for Research and Technology

Janelle R. Thompson

Nanyang Technological University (NTU) - Singapore Centre for Environmental Life Sciences Engineering (SCELSE)

Eng Eong Ooi

Duke-NUS Graduate Medical School - Programme in Emerging Infectious Diseases

Jenny G. Low

National University of Singapore (NUS) - Department of Infectious Diseases

Eric J. Alm

Singapore-MIT Alliance for Research and Technology - Antimicrobial Resistance Interdisciplinary Research Group

Shirin Kalimuddin

National University of Singapore (NUS) - Department of Infectious Diseases; Duke NUS Medical School - Emerging Infectious Disease Program

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Abstract

Antibiotic-associated diarrhea (AAD) affects a significant proportion of patients receiving antibiotics. We sought to understand if differences in the gut microbiome would influence the development of AAD. We administered a 3-day course of amoxicillin-clavulanate to 30 healthy adult volunteers, and analyzed their stool microbiome, using 16S rRNA gene sequencing, at baseline and up to 4-weeks post-antibiotic administration. Lower levels of gut Ruminococcaceae were significantly and consistently observed from baseline till Day 7 in participants who developed AAD. Overall, participants who developed AAD experienced a greater decrease in microbial diversity. The probability of AAD could be predicted based on qPCR-derived levels of Faecalibacterium prausnitzii . Our findings suggest that a lack of gut Ruminococcaceae influences development of AAD. Quantification of F. prausnitzii in stool prior to antibiotic administration may help identify patients at risk of AAD, and aid clinicians in devising individualised treatment regimens to minimise such adverse effects.

Keywords: Antibiotic-associated diarrhea (AAD), amoxicillin-clavulanate, Gut microbiome, Ruminococcaceae, F. prausnitzii

Suggested Citation

Gu, Xiaoqiong and Sim, Jean XY and Lee, Wei Lin and Cui, Liang and Chan, Yvonne FZ and Chang, Ega Danu and Teh, Yii Ean and Zhang, An-Ni and Armas, Federica and Chandra, Franciscus and Hongjie, Chen and Zhao, Shijie and Lee, Zhanyi and Thompson, Janelle R. and Ooi, Eng Eong and Low, Jenny G. and Alm, Eric J. and Kalimuddin, Shirin, Gut Ruminococcaceae Levels Correlate with Risk of Antibiotic-Associated Diarrhea. Available at SSRN: https://ssrn.com/abstract=3942852 or http://dx.doi.org/10.2139/ssrn.3942852
This version of the paper has not been formally peer reviewed.

Xiaoqiong Gu

Singapore-MIT Alliance for Research and Technology ( email )

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Singapore
Singapore

Jean XY Sim

National University of Singapore (NUS) - Department of Infection Prevention and Epidemiology ( email )

Wei Lin Lee

Singapore-MIT Alliance for Research and Technology - Antimicrobial Resistance Interdisciplinary Research Group ( email )

Singapore

Liang Cui

Singapore-MIT Alliance for Research and Technology ( email )

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Singapore
Singapore

Yvonne FZ Chan

National University of Singapore (NUS) - Department of Infectious Diseases ( email )

Singapore

Ega Danu Chang

Singapore-MIT Alliance for Research and Technology - Antimicrobial Resistance Interdisciplinary Research Group ( email )

Singapore

Yii Ean Teh

National University of Singapore (NUS) - Department of Infectious Diseases ( email )

Singapore

An-Ni Zhang

Massachusetts Institute of Technology (MIT) - Department of Biological Engineering ( email )

77 Massachusetts Avenue
50 Memorial Drive
Cambridge, MA 02139-4307
United States

Federica Armas

Singapore-MIT Alliance for Research and Technology - Antimicrobial Resistance Interdisciplinary Research Group ( email )

Singapore

Franciscus Chandra

Singapore-MIT Alliance for Research and Technology - Antimicrobial Resistance Interdisciplinary Research Group ( email )

Singapore

Chen Hongjie

Singapore-MIT Alliance for Research and Technology - Antimicrobial Resistance Interdisciplinary Research Group ( email )

Singapore

Shijie Zhao

Massachusetts Institute of Technology (MIT) - Department of Biological Engineering ( email )

77 Massachusetts Avenue
50 Memorial Drive
Cambridge, MA 02139-4307
United States

Zhanyi Lee

Singapore-MIT Alliance for Research and Technology ( email )

1Create way
Singapore
Singapore

Janelle R. Thompson

Nanyang Technological University (NTU) - Singapore Centre for Environmental Life Sciences Engineering (SCELSE) ( email )

Singapore

Eng Eong Ooi

Duke-NUS Graduate Medical School - Programme in Emerging Infectious Diseases ( email )

Singapore

Jenny G. Low

National University of Singapore (NUS) - Department of Infectious Diseases ( email )

Singapore

Eric J. Alm

Singapore-MIT Alliance for Research and Technology - Antimicrobial Resistance Interdisciplinary Research Group ( email )

Singapore

Shirin Kalimuddin (Contact Author)

National University of Singapore (NUS) - Department of Infectious Diseases

Singapore

Duke NUS Medical School - Emerging Infectious Disease Program

Singapore

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