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The Dynamic Composition of an Archetypal Plant Condensate Highlights a Tug-of-War between Condensates and Cell Vertex

87 Pages Posted: 15 Oct 2021 Publication Status: Review Complete

See all articles by Chen Liu

Chen Liu

Swedish University of Agricultural Sciences (SLU) - Department of Plant Biology

Andriani Mentzelopoulou

University of Crete - Department of Biology

Ioannis H. Hatzianestis

University of Crete - Department of Biology

Rafail Gkritzas

University of Crete - Department of Biology

Amna Muhammad

Swedish University of Agricultural Sciences (SLU) - Department of Plant Biology

Francisco J. Romero-Campero

University of Seville - Department of Computer Science and Artificial Intelligence

Ana B. Romero-Losada

University of Seville - Department of Computer Science and Artificial Intelligence

Emilio Gutierrez-Beltran

University of Seville - Institute of Plant Biochemistry and Photosynthesis

Panagiotis Moschou

Foundation for Research and Technology - Hellas (FORTH); Swedish University of Agricultural Sciences (SLU) - Department of Plant Biology

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Abstract

Cellular condensates are usually ribonucleoprotein membrane-less assemblies that can shuttle between liquid or less fluid states. These phase transitions could impact a broad range of biological outcomes. Here, by developing proximity-biotinylation approaches, we unveil the composition of the condensate known as processing bodies (PBs) in the model plant Arabidopsis. We show that PBs comprise both conserved and dynamic networks with unexpected connections to membrane subdomains. We study further one of these networks and discover a cell vertex-defining module with membrane remodeling properties. This module functions through a liquid/solid-like phase transition that impacts growth. The underlying mechanism is developmentally regulated by a tug-of-war between PBs and the plasma membrane instructed by a phospho-switch. We thus provide a framework for the elucidation of condensates in plants and describe a growth module based on phase transitions of condensates at a membranous interface.

Keywords: Biomolecular condensates, Phase separation, Developmental patterning, Plasma membrane pattern, Cytoskeleton, Vesicle trafficking, Cell Polarity

Suggested Citation

Liu, Chen and Mentzelopoulou, Andriani and Hatzianestis, Ioannis H. and Gkritzas, Rafail and Muhammad, Amna and Romero-Campero, Francisco J. and Romero-Losada, Ana B. and Gutierrez-Beltran, Emilio and Moschou, Panagiotis, The Dynamic Composition of an Archetypal Plant Condensate Highlights a Tug-of-War between Condensates and Cell Vertex. Available at SSRN: https://ssrn.com/abstract=3943616 or http://dx.doi.org/10.2139/ssrn.3943616
This version of the paper has not been formally peer reviewed.

Chen Liu

Swedish University of Agricultural Sciences (SLU) - Department of Plant Biology ( email )

Sweden

Andriani Mentzelopoulou

University of Crete - Department of Biology ( email )

Heraklion
Greece

Ioannis H. Hatzianestis

University of Crete - Department of Biology ( email )

Heraklion
Greece

Rafail Gkritzas

University of Crete - Department of Biology ( email )

Heraklion
Greece

Amna Muhammad

Swedish University of Agricultural Sciences (SLU) - Department of Plant Biology ( email )

Sweden

Francisco J. Romero-Campero

University of Seville - Department of Computer Science and Artificial Intelligence ( email )

Seville
Spain

Ana B. Romero-Losada

University of Seville - Department of Computer Science and Artificial Intelligence ( email )

Seville
Spain

Emilio Gutierrez-Beltran

University of Seville - Institute of Plant Biochemistry and Photosynthesis ( email )

Sevilla
Spain

Panagiotis Moschou (Contact Author)

Foundation for Research and Technology - Hellas (FORTH)

Crete
Greece

Swedish University of Agricultural Sciences (SLU) - Department of Plant Biology ( email )

Sweden

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