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Long Noncoding RNA LINC01435 Impedes Diabetic Wound Healing by Facilitating YY1-Mediated HDAC8 Expression

51 Pages Posted: 27 Oct 2021 Publication Status: Published

See all articles by Wan Fu

Wan Fu

Sun Yat-Sen University (SYSU) - Department of Endocrinology

Diefei Liang

Sun Yat-Sen University (SYSU) - Department of Endocrinology

Xiaoying Wu

Sun Yat-Sen University (SYSU) - Department of Endocrinology

Hongxing Chen

Sun Yat-Sen University (SYSU) - Department of Endocrinology

Xiaosi Hong

Sun Yat-Sen University (SYSU) - Department of Endocrinology

Jia huan Wang

Sun Yat-Sen University (SYSU) - Department of Endocrinology

Tianxin Zhu

Sun Yat-Sen University (SYSU) - Department of Endocrinology

Tingting Zeng

Sun Yat-Sen University (SYSU) - Department of Endocrinology

Weijie Lin

Sun Yat-Sen University - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation

Sifan Chen

Sun Yat-Sen University - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation

Li Yan

Sun Yat-Sen University (SYSU) - Department of Endocrinology

Meng Ren

Sun Yat-Sen University (SYSU) - Department of Endocrinology

More...

Abstract

Abnormal interactions between skin cells play an important role in the dysregulation of diabetic wound recovery. Exosomes are cell-derived lipid nanoparticles that transport messages between cells, and isolating and identifying potential therapeutic noncoding RNAs from exosomes is very important. We demonstrated that treatment with Exos from high glucose-pretreated immortalized human epidermal (HaCaT) cells (HG-Exos) could delay the wound healing process in diabetic mice. Further analysis indicated the Exo-mediated uptake of LINC01435 in recipient human umbilical vein endothelial cells (HUVECs) changes the subcellular localization of the transcription factor Yin Yang 1 (YY1) and cooperates with YY1 to upregulate the expression of histone deacetylases (HDACs)8, resulting in decreased tube formation and ability of HUVECs to migrate, thus angiogenesis was inhibited. These results suggest that LINC01435/YY1/HDAC8 may be an important signaling pathway affecting the recovery of diabetic wounds, which makes it a potential target for the treatment of diabetic foot ulcers.

Keywords: Angiogenesis, Diabetic wound healing, Exosomes, LncRNAs

Suggested Citation

Fu, Wan and Liang, Diefei and Wu, Xiaoying and Chen, Hongxing and Hong, Xiaosi and Wang, Jia huan and Zhu, Tianxin and Zeng, Tingting and Lin, Weijie and Chen, Sifan and Yan, Li and Ren, Meng, Long Noncoding RNA LINC01435 Impedes Diabetic Wound Healing by Facilitating YY1-Mediated HDAC8 Expression. Available at SSRN: https://ssrn.com/abstract=3951497 or http://dx.doi.org/10.2139/ssrn.3951497
This version of the paper has not been formally peer reviewed.

Wan Fu

Sun Yat-Sen University (SYSU) - Department of Endocrinology

135, Xingang Xi Road
Haizhu District
Guangzhou, Guangdong 510275
China

Diefei Liang

Sun Yat-Sen University (SYSU) - Department of Endocrinology

135, Xingang Xi Road
Haizhu District
Guangzhou, Guangdong 510275
China

Xiaoying Wu

Sun Yat-Sen University (SYSU) - Department of Endocrinology

135, Xingang Xi Road
Haizhu District
Guangzhou, Guangdong 510275
China

Hongxing Chen

Sun Yat-Sen University (SYSU) - Department of Endocrinology

135, Xingang Xi Road
Haizhu District
Guangzhou, Guangdong 510275
China

Xiaosi Hong

Sun Yat-Sen University (SYSU) - Department of Endocrinology

135, Xingang Xi Road
Haizhu District
Guangzhou, Guangdong 510275
China

Jia huan Wang

Sun Yat-Sen University (SYSU) - Department of Endocrinology ( email )

Guangzhou, Guangdong 510120
China

Tianxin Zhu

Sun Yat-Sen University (SYSU) - Department of Endocrinology

135, Xingang Xi Road
Haizhu District
Guangzhou, Guangdong 510275
China

Tingting Zeng

Sun Yat-Sen University (SYSU) - Department of Endocrinology

135, Xingang Xi Road
Haizhu District
Guangzhou, Guangdong 510275
China

Weijie Lin

Sun Yat-Sen University - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation

135, Xingang Xi Road
Haizhu District
Guangzhou, Guangdong 510275
China

Sifan Chen

Sun Yat-Sen University - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation

Guangzhou
China

Li Yan

Sun Yat-Sen University (SYSU) - Department of Endocrinology

135, Xingang Xi Road
Haizhu District
Guangzhou, Guangdong 510275
China

Meng Ren (Contact Author)

Sun Yat-Sen University (SYSU) - Department of Endocrinology ( email )

Guangzhou, Guangdong 510120
China

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