Oxidative Status of Planarians is Differently Affected by PAHs: 3-5 Benzene Ring Compounds

34 Pages Posted: 27 Nov 2021

See all articles by Fátima C. P. Simão

Fátima C. P. Simão

Universidade de Aveiro - Centre for Environmental and Marine Studies (CESAM)

Andreia C. M. Rodrigues

Universidade de Aveiro - Centre for Environmental and Marine Studies (CESAM)

Carlos Gravato

University of Lisbon - Centre for Environmental and Marine Studies (CESAM)

Amadeu M.V.M. Soares

Universidade de Aveiro - Department of Biology

João L.T. Pestana

Universidade de Aveiro - Centre for Environmental and Marine Studies (CESAM)

Abstract

Planarians are promising model animals and are increasing in importance for ecotoxicological studies. However, there is scarce to no information for many contaminants, such as polycyclic aromatic hydrocarbons (PAHs), regarding their specific toxicity towards planarians. We aimed to uncover how vital physiological functions, such as, neurophysiology, detoxification and oxidative stress status, and cellular energy allocation are affected on the freshwater planarian Girardia tigrina by low and high molecular weight PAHs – phenanthrene, pyrene and benzo[a]pyrene (B[a]P). Phenanthrene affected planarian antioxidant defences and detoxification, increasing glutathione levels and activity of glutathione-S-transferase (GST), the latter in a dose-dependent way (from 75 to 300 µg L-1). Phenanthrene also decreased carbohydrates in planarians exposed to 150 and 300 µg L-1. Pyrene caused higher oxygen consumption and decreased energy budget in planarians exposed to 75 and 150 µg L-1. B[a]P exposure decreased G. tigrina GST activity in a dose-dependent way. Lower lipid peroxidation levels were measured in planarians exposed to 2.5 and 75 µg L-1 of pyrene and at 37.5, 75 and 150 µg L-1 of B[a]P. The present data indicate that each of these PAHs seems to elicit distinct biochemical responses in planarians, possibly pointing to different biochemical interactions and/or metabolisation of each compound.

Keywords: Epibenthic freshwater planarians, polycyclic aromatic hydrocarbons, oxidative stress status, energy budget, lipid peroxidation

Suggested Citation

Simão, Fátima C. P. and Rodrigues, Andreia C. M. and Gravato, Carlos and Soares, Amadeu M.V.M. and Pestana, João L.T., Oxidative Status of Planarians is Differently Affected by PAHs: 3-5 Benzene Ring Compounds. Available at SSRN: https://ssrn.com/abstract=3972656 or http://dx.doi.org/10.2139/ssrn.3972656

Fátima C. P. Simão

Universidade de Aveiro - Centre for Environmental and Marine Studies (CESAM) ( email )

Aveiro
Portugal

Andreia C. M. Rodrigues (Contact Author)

Universidade de Aveiro - Centre for Environmental and Marine Studies (CESAM) ( email )

Aveiro
Portugal

Carlos Gravato

University of Lisbon - Centre for Environmental and Marine Studies (CESAM) ( email )

Campo Grande
Portugal

Amadeu M.V.M. Soares

Universidade de Aveiro - Department of Biology ( email )

João L.T. Pestana

Universidade de Aveiro - Centre for Environmental and Marine Studies (CESAM) ( email )

Aveiro
Portugal

Do you have a job opening that you would like to promote on SSRN?

Paper statistics

Downloads
25
Abstract Views
183
PlumX Metrics