Emerging Elisa Derived Technologies for in Vitro  Diagnostics

Abstract

ELISA technology has received great attention in in vitro  diagnosis due to its high specificity, low cost and high throughput. However, the traditional ELISA technology is tedious, lack of multiple detection capabilities, and is not easy to implement in point-of-care (POC) settings. The emergence and development of magnetic bead technology, paper-based platform and nanomaterials have attracted researchers in various fields to improve and design ELISA. Recently, ELISA has been innovatively used to associate with these analytical techniques to confer high sensitivity, specificity, repeatability, low cost and multiple detection capabilities. However, the applications of various ELISA derived technologies in in vitro  diagnosis are rarely reviewed. In this review, we first introduced the history and principle related to ELISA technology, then we classify and introduce various ELISA derived technologies with an emphasis, and finally we discuss the current challenges and prospects of ELISA technology.

Keywords: immunoassay, in vitro diagnostics, novel solid substrate, novel recognition element, signal amplification strategy