Mining-Related Multi-Resistance Genes in Typical Karst Nonferrous Metal(Loid) Tailing Impoundments Under Bio-Restoration

33 Pages Posted: 29 Dec 2021

See all articles by Jianli Liu

Jianli Liu

China University of Geosciences (CUG) - School of Water Resources and Environment and Research Center of Environmental Science and Engineering

Jun Yao

China University of Geosciences (CUG) - School of Water Resources and Environment and Research Center of Environmental Science and Engineering

Deliang Zhou

Beijing Zhongdian Yida Technology Co., Ltd.

Hao Li

China University of Geosciences (CUG) - School of Water Resources and Environment and Research Center of Environmental Science and Engineering

Bang Liu

China University of Geosciences (CUG) - School of Water Resources and Environment and Research Center of Environmental Science and Engineering

Weihua Cui

China University of Geosciences (CUG) - School of Water Resources and Environment and Research Center of Environmental Science and Engineering

Xinyuan Li

China University of Geosciences (CUG) - School of Water Resources and Environment and Research Center of Environmental Science and Engineering

Hang Meng

China University of Geosciences (CUG) - School of Water Resources and Environment and Research Center of Environmental Science and Engineering

Robert Duran

China University of Geosciences (CUG) - School of Water Resources and Environment and Research Center of Environmental Science and Engineering

Abstract

Tailing impoundments as reservoirs of multi-resistance posed a threat to human health. Potential co-selection of antibiotic resistance genes (ARGs) with metal(loid) resistance genes (MRGs) during bio-restoration have been poorly investigated. Here we show that abundance of sulfonamide-related ARGs based on Metagenomic analysis decreased, whereas those related to bacitracin and chloramphenicol increased. Metal(loid)s contents (including As, Cd, Fe, Pb, Sb, and Zn) were the main drivers for microbial organization. Thiobacillus and Sphingomonas being the main genera carrying the bacA and mexef resistance operons, which were also found as main genera carrying MRGs together with Sulfuricella genus. The added SRB strains Desulfosporosinus and Desulfotomaculum, and acidophilic strain Acidiphilium significantly contributed to the distribution of sul genes, while Thiobacillus, Sphingomonas, Sulfuricella, and Meiothermus genera significantly contributed to the distribution of MRGs. Additionally, ARGs (mexNW, merD, sul and bla) and MRGs (czcABCR and copRS genes) were found on the same contig, revealing the co-selection of ARGs and MRGs. Co-selection of ARGs and MRGs should be considered for the implementation bio-restoration processes as they can impact human health. The study also shows the involvement of iron metabolism via Feo-like genes in bio-restoration process, providing new insights, useful for the management of tailings’ bio-restoration.

Keywords: Microorganism, Antimicrobial resistance, Metal resistance, Co-selection, Tailings impoundments

Suggested Citation

Liu, Jianli and Yao, Jun and Zhou, Deliang and Li, Hao and Liu, Bang and Cui, Weihua and Li, Xinyuan and Meng, Hang and Duran, Robert, Mining-Related Multi-Resistance Genes in Typical Karst Nonferrous Metal(Loid) Tailing Impoundments Under Bio-Restoration. Available at SSRN: https://ssrn.com/abstract=3996109 or http://dx.doi.org/10.2139/ssrn.3996109

Jianli Liu

China University of Geosciences (CUG) - School of Water Resources and Environment and Research Center of Environmental Science and Engineering ( email )

29 Xueyuan Road
Beijing, 100083
China

Jun Yao (Contact Author)

China University of Geosciences (CUG) - School of Water Resources and Environment and Research Center of Environmental Science and Engineering ( email )

29 Xueyuan Road
Beijing, 100083
China

Deliang Zhou

Beijing Zhongdian Yida Technology Co., Ltd. ( email )

Beijing
China

Hao Li

China University of Geosciences (CUG) - School of Water Resources and Environment and Research Center of Environmental Science and Engineering ( email )

29 Xueyuan Road
Beijing, 100083
China

Bang Liu

China University of Geosciences (CUG) - School of Water Resources and Environment and Research Center of Environmental Science and Engineering ( email )

29 Xueyuan Road
Beijing, 100083
China

Weihua Cui

China University of Geosciences (CUG) - School of Water Resources and Environment and Research Center of Environmental Science and Engineering ( email )

29 Xueyuan Road
Beijing, 100083
China

Xinyuan Li

China University of Geosciences (CUG) - School of Water Resources and Environment and Research Center of Environmental Science and Engineering ( email )

29 Xueyuan Road
Beijing, 100083
China

Hang Meng

China University of Geosciences (CUG) - School of Water Resources and Environment and Research Center of Environmental Science and Engineering ( email )

29 Xueyuan Road
Beijing, 100083
China

Robert Duran

China University of Geosciences (CUG) - School of Water Resources and Environment and Research Center of Environmental Science and Engineering ( email )

29 Xueyuan Road
Beijing, 100083
China

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