Determination pk a Values of Six Phenolic Acids by Pressure-Assisted Capillary Electrophoresis and Simultaneous Determination of These Six Phenolic Acids in Euphorbiae Lathyris L. By Capillary Zone Electrophoresis with Heptapkis (2,6-di-o-Methyl)-β-Cyclodextrin as Modifier

24 Pages Posted: 14 Jan 2022

See all articles by Meijuan Hou

Meijuan Hou

Shanxi Medical University

Wenyu Xie

Shanxi Medical University

Yanping Yu

Shanxi Medical University

Yiqiong Zhang

Shanxi Medical University

Guangbin Zhang

Shanxi Medical University

Haixia Yu

Shanxi Medical University

Hongfen Zhang

Shanxi Medical University; Shanxi Medical University - School of Pharmacy

Ruimiao Chang

Shanxi Medical University

Anjia Chen

Shanxi Medical University - School of Pharmacy

Abstract

The purpose of this study was to determine the pΚa values of six phenolic acids (Chlorogenic acid, Ferulic acid, p-Coumaric acid, Vanillic acid, Caffeic acid and Gallic acid) by pressure-assisted capillary electrophoresis and develop a capillary zone electrophoresis method (CZE) for simultaneous separation and determination of these six phenolic acids in Euphorbiae Lathyris L. The measured pΚa values for the six phenolic acids were Chlorogenic acid (3.96), Ferulic acid (4.57), p-Coumaric acid (4.65), Vanillic acid (4.56), Caffeic acid (4.63) and Gallic acid (4.40), respectively. These six analytes were baseline separated within 15 minutes with a running buffer consisting of 35 mM sodium tetraborate and 3.0 mM Heptapkis (2,6-di-o-Methyl)-β-cyclodextrin (DM-β-CD) in water-methanol (95:5, v/v) (pH=9.18). The applied voltage, capillary temperature and detection wavelength were 12.5 kV, 25°C and 260 nm, respectively. Under the optimum conditions, good linearities were achieved with correlation coefficients from 0.9925 to 0.9990. The detection limits and the quantitation limits ranged from 0.08 to 0.65 μg/mL and from 0.30 to 1.95 μg/mL, respectively. Excellent accuracy and precision were obtained. Recoveries of the analytes varied from 98.2% to 104.9%. This established method was novel, simple, rapid, and it was successfully used in the analysis of these phenolic acids in Euphorbiae Lathyris L. with relatively simple extraction procedures, the assay results were satisfactory.

Keywords: Euphorbiae Lathyris L., acid-base dissociation constants, pressure-assisted capillary electrophoresis, Heptapkis (2, 6-di-o-Methyl)-β-cyclodextrin

Suggested Citation

Hou, Meijuan and Xie, Wenyu and Yu, Yanping and Zhang, Yiqiong and Zhang, Guangbin and Yu, Haixia and Zhang, Hongfen and Chang, Ruimiao and Chen, Anjia, Determination pk a Values of Six Phenolic Acids by Pressure-Assisted Capillary Electrophoresis and Simultaneous Determination of These Six Phenolic Acids in Euphorbiae Lathyris L. By Capillary Zone Electrophoresis with Heptapkis (2,6-di-o-Methyl)-β-Cyclodextrin as Modifier. Available at SSRN: https://ssrn.com/abstract=4008858 or http://dx.doi.org/10.2139/ssrn.4008858

Meijuan Hou

Shanxi Medical University ( email )

Taiyuan
China

Wenyu Xie

Shanxi Medical University ( email )

Taiyuan
China

Yanping Yu

Shanxi Medical University ( email )

Taiyuan
China

Yiqiong Zhang

Shanxi Medical University ( email )

Taiyuan
China

Guangbin Zhang

Shanxi Medical University ( email )

Taiyuan
China

Haixia Yu

Shanxi Medical University ( email )

Taiyuan
China

Hongfen Zhang

Shanxi Medical University ( email )

Taiyuan
China

Shanxi Medical University - School of Pharmacy ( email )

Jinzhong, Shanxi 030619
China

Ruimiao Chang

Shanxi Medical University ( email )

Taiyuan
China

Anjia Chen (Contact Author)

Shanxi Medical University - School of Pharmacy ( email )

Jinzhong, Shanxi 030619
China

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