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Characterization of Bombali Virus, a New Bat Filovirus

47 Pages Posted: 15 Feb 2022 Publication Status: Review Complete

See all articles by Laura McMullan

Laura McMullan

Government of the United States of America - Viral Special Pathogens Branch

Mike Flint

Government of the United States of America - Viral Special Pathogens Branch

M. Harley Jenks

Government of the United States of America - Viral Special Pathogens Branch

Lisa W. Guerrero

Government of the United States of America - Viral Special Pathogens Branch

Ayan K. Chakrabarti

Government of the United States of America - Viral Special Pathogens Branch

Cynthia Goldsmith

Government of the United States of America - Infectious Diseases Pathology Branch

Jonathan C. Guito

Government of the United States of America - Infectious Diseases Pathology Branch

Tara K. Sealy

Government of the United States of America - Viral Special Pathogens Branch

JoAnn D. Coleman-McCray

Government of the United States of America - Viral Special Pathogens Branch

Stephen R. Welch

Government of the United States of America - Viral Special Pathogens Branch

Jessica R. Spengler

Government of the United States of America - Viral Special Pathogens Branch

Gustavo Palacios

US Army Medical Research Institute of Infectious Diseases

Brian Bird

University of California - One Health Institute

Tracey Goldstein

University of California - One Health Institute

Jonna AK Mazet

University of California - One Health Institute

Danielle Porter

Gilead Sciences

Zachary A. Bornholdt

Mapp Biopharmaceutical Inc.

Larry Zeitlin

Mapp Biopharmaceutical Inc.

Sherif R. Zaki

Government of the United States of America - Infectious Diseases Pathology Branch

Stuart T. Nichol

Government of the United States of America - Viral Special Pathogens Branch

Joel M. Montgomery

Government of the United States of America - Viral Special Pathogens Branch

Jonathan S. Towner

Government of the United States of America - Viral Special Pathogens Branch

Christina F. Spiropoulou

Government of the United States of America - Viral Special Pathogens Branch

Cesar Albarino

Government of the United States of America - Viral Special Pathogens Branch

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Abstract

Bombali virus (BOMV), a new ebolavirus, has been genetically identified in insectivorous bats Mops condylurus and Chaerephon pumilus in equatorial Africa. Filoviruses cause recurrent outbreaks of severe, high-mortality disease in humans. To characterize and assess BOMV’s potential virulence, we isolated infectious virus using reverse genetics. We found BOMV morphologically indistinguishable from Ebola virus (EBOV), infected human cells and primary human macrophages, and efficiently used M. condylurus Niemann-Pick C1 (NPC1) over human NPC1 to enter cells. We found functional complementation between BOMV and EBOV replicase proteins using human host factors in a minigenome system. Both BOMV and EBOV induced proinflammatory genes while inhibiting expression of key macrophage receptors important to controlling phagocytosis and antigen presentation. Unlike EBOV, however, BOMV failed to induce an antiviral response. Here, we provide foundational characterization of a new bat-borne ebolavirus and its potential as a human pathogen.

Keywords: Bombali virus, Ebola virus, NPC1, interferon antagonist, antiviral and monoclonal therapeutics, reverse genetics, host transcription and pathway analysis, bat viruses

Suggested Citation

McMullan, Laura and Flint, Mike and Jenks, M. Harley and Guerrero, Lisa W. and Chakrabarti, Ayan K. and Goldsmith, Cynthia and Guito, Jonathan C. and Sealy, Tara K. and Coleman-McCray, JoAnn D. and Welch, Stephen R. and Spengler, Jessica R. and Palacios, Gustavo and Bird, Brian and Goldstein, Tracey and Mazet, Jonna AK and Porter, Danielle and Bornholdt, Zachary A. and Zeitlin, Larry and Zaki, Sherif R. and Nichol, Stuart T. and Montgomery, Joel M. and Towner, Jonathan S. and Spiropoulou, Christina F. and Albarino, Cesar, Characterization of Bombali Virus, a New Bat Filovirus. Available at SSRN: https://ssrn.com/abstract=4035855 or http://dx.doi.org/10.2139/ssrn.4035855
This version of the paper has not been formally peer reviewed.

Laura McMullan (Contact Author)

Government of the United States of America - Viral Special Pathogens Branch ( email )

Atlanta, GA 30329
United States

Mike Flint

Government of the United States of America - Viral Special Pathogens Branch ( email )

Atlanta, GA 30329
United States

M. Harley Jenks

Government of the United States of America - Viral Special Pathogens Branch ( email )

Atlanta, GA 30329
United States

Lisa W. Guerrero

Government of the United States of America - Viral Special Pathogens Branch ( email )

Atlanta, GA 30329
United States

Ayan K. Chakrabarti

Government of the United States of America - Viral Special Pathogens Branch ( email )

Atlanta, GA 30329
United States

Cynthia Goldsmith

Government of the United States of America - Infectious Diseases Pathology Branch ( email )

Atlanta, GA 30329
United States

Jonathan C. Guito

Government of the United States of America - Infectious Diseases Pathology Branch ( email )

Atlanta, GA 30329
United States

Tara K. Sealy

Government of the United States of America - Viral Special Pathogens Branch ( email )

Atlanta, GA 30329
United States

JoAnn D. Coleman-McCray

Government of the United States of America - Viral Special Pathogens Branch ( email )

Atlanta, GA 30329
United States

Stephen R. Welch

Government of the United States of America - Viral Special Pathogens Branch ( email )

Atlanta, GA 30329
United States

Jessica R. Spengler

Government of the United States of America - Viral Special Pathogens Branch ( email )

Atlanta, GA 30329
United States

Gustavo Palacios

US Army Medical Research Institute of Infectious Diseases ( email )

Frederick, MD 21702
United States

Brian Bird

University of California - One Health Institute ( email )

Davis, CA 95616
United States

Tracey Goldstein

University of California - One Health Institute ( email )

Davis, CA 95616
United States

Jonna AK Mazet

University of California - One Health Institute ( email )

Davis, CA 95616
United States

Danielle Porter

Gilead Sciences ( email )

Foster City, CA 94404
United States

Zachary A. Bornholdt

Mapp Biopharmaceutical Inc. ( email )

San Diego, CA 92121
United States

Larry Zeitlin

Mapp Biopharmaceutical Inc. ( email )

San Diego, CA 92121
United States

Sherif R. Zaki

Government of the United States of America - Infectious Diseases Pathology Branch ( email )

Atlanta, GA 30329
United States

Stuart T. Nichol

Government of the United States of America - Viral Special Pathogens Branch ( email )

Atlanta, GA 30329
United States

Joel M. Montgomery

Government of the United States of America - Viral Special Pathogens Branch ( email )

Atlanta, GA 30329
United States

Jonathan S. Towner

Government of the United States of America - Viral Special Pathogens Branch ( email )

Atlanta, GA 30329
United States

Christina F. Spiropoulou

Government of the United States of America - Viral Special Pathogens Branch ( email )

Atlanta, GA 30329
United States

Cesar Albarino

Government of the United States of America - Viral Special Pathogens Branch ( email )

Atlanta, GA 30329
United States

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