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Bioprinting of Hair Follicle Germs for Hair Regenerative Medicine

32 Pages Posted: 3 Mar 2022 Publication Status: Accepted

See all articles by Ayaka Nanmo

Ayaka Nanmo

Yokohama National University - Faculty of Engineering

Lei Yan

Yokohama National University - Faculty of Engineering

Tomoki Asaba

Yokohama National University - Faculty of Engineering

Licheng Wan

Yokohama National University - Faculty of Engineering

Tatsuto Kageyama

Yokohama National University - Faculty of Engineering

Junji Fukuda

Yokohama National University - Faculty of Engineering

Abstract

Hair regenerative medicine is a promising approach to treat hair loss. The replication of in vivo tissue configurations and microenvironments, such as hair follicle germs, has been studied to prepare tissue grafts for hair regenerative medicine. However, such approaches should be scalable, because a single patient with alopecia requires thousands of tissue grafts. In this paper, we propose an approach for the scalable and automated preparation of highly hair-inductive tissue grafts using a bioprinter. Two collagen droplets (2 µL each) containing mesenchymal and epithelial cells were placed adjacent to each other to fabricate hair-follicle-germ-like grafts. During three days of culture, the pairs of microgel beads were spontaneously contracted by cell traction forces, whereas the two cell types remained separated, where the densities of the cells and collagen were enriched more than 10 times. This approach allowed us to fabricate submillimeter objects printed with millimeter-order accuracy, facilitating scalable and automated tissue graft preparation. Because of mesenchymal–epithelial interactions, hair microgels (HMGs, i.e., collagen- and cell-enriched microgels) efficiently regenerate hair follicles and shafts when transplanted into the back skin of mice. However, the generated hair shafts mostly remain under the skin. Therefore, we printed microgels onto surgical suture guides arrayed on a stage. The microgels were contracted along with the suture guides in culture prior to transplantation. The guide-inserted HMGs significantly improved hair-shaft sprouting through the skin, owing to the control of the orientation of the HMGs transplanted into the skin. This approach is a promising strategy to advance hair regenerative medicine.

Keywords: bioprinting, hair regeneration, hair follicle germ, microgel, collagen contraction

Suggested Citation

Nanmo, Ayaka and Yan, Lei and Asaba, Tomoki and Wan, Licheng and Kageyama, Tatsuto and Fukuda, Junji, Bioprinting of Hair Follicle Germs for Hair Regenerative Medicine. Available at SSRN: https://ssrn.com/abstract=4048683 or http://dx.doi.org/10.2139/ssrn.4048683

Ayaka Nanmo

Yokohama National University - Faculty of Engineering ( email )

Yokohama, Kanagawa
Japan

Lei Yan

Yokohama National University - Faculty of Engineering ( email )

Yokohama, Kanagawa
Japan

Tomoki Asaba

Yokohama National University - Faculty of Engineering ( email )

Yokohama, Kanagawa
Japan

Licheng Wan

Yokohama National University - Faculty of Engineering ( email )

Yokohama, Kanagawa
Japan

Tatsuto Kageyama

Yokohama National University - Faculty of Engineering ( email )

Yokohama, Kanagawa
Japan

Junji Fukuda (Contact Author)

Yokohama National University - Faculty of Engineering ( email )

Yokohama, Kanagawa
Japan

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