puc-header

Rapid Detection of Staphylococcus aureus and Streptococcus pneumoniae by Real-Time Analysis of Volatile Metabolites

76 Pages Posted: 18 Apr 2022 Publication Status: Published

See all articles by Alejandro Gómez-Mejia

Alejandro Gómez-Mejia

University of Zurich - Division of Infectious Diseases and Hospital Epidemiology

Arnold Kim

University Children’s Hospital Basel (UKBB)

Bär Julian

University of Zurich - Division of Infectious Diseases and Hospital Epidemiology

Kapil Dev Singh

University Children’s Hospital Basel (UKBB)

Thomas C. Scheier

University of Zurich - Division of Infectious Diseases and Hospital Epidemiology

Silvio D. Brugger

University of Zurich - Division of Infectious Diseases and Hospital Epidemiology

Annelies S. Zinkernagel

University of Zurich - Division of Infectious Diseases and Hospital Epidemiology

Pablo Sinues

University Children’s Hospital Basel (UKBB)

More...

Abstract

Early detection of pathogenic bacteria is needed for rapid diagnostics allowing adequate and timely treatment of infections. In this study, we show that Secondary Electro-Spray Ionization-High Resolution Mass Spectrometry (SESI-HRMS) can be used as a diagnostic tool for rapid detection of bacterial infections as a supportive system for current state of the art diagnostics. Volatile Organic Compounds (VOCs) produced by growing S. aureus or S. pneumoniae cultures on blood agar plates were detected within minutes and allowed for the distinction of these two bacteria on a species and even strain level within hours. Furthermore, we obtained a fingerprint of clinical patient samples within minutes of measurement and predominantly observed a separation of samples containing living bacteria compared to samples with no bacterial growth. Further development of this technique may reduce the time required for microbiological diagnosis and should help to improve patient’s tailored treatment.

Keywords: bacterial infections; clinical diagnostics; real-time mass spectrometry; SESI-HRMS; Staphylococcus aureus; Streptococcus pneumoniae

Suggested Citation

Gómez-Mejia, Alejandro and Kim, Arnold and Julian, Bär and Singh, Kapil Dev and Scheier, Thomas C. and Brugger, Silvio D. and Zinkernagel, Annelies S. and Sinues, Pablo, Rapid Detection of Staphylococcus aureus and Streptococcus pneumoniae by Real-Time Analysis of Volatile Metabolites. Available at SSRN: https://ssrn.com/abstract=4086944 or http://dx.doi.org/10.2139/ssrn.4086944
This version of the paper has not been formally peer reviewed.

Alejandro Gómez-Mejia

University of Zurich - Division of Infectious Diseases and Hospital Epidemiology ( email )

Raemistrasse 100
Zürich, 8091
Switzerland

Arnold Kim

University Children’s Hospital Basel (UKBB) ( email )

Bär Julian

University of Zurich - Division of Infectious Diseases and Hospital Epidemiology ( email )

Kapil Dev Singh

University Children’s Hospital Basel (UKBB) ( email )

Thomas C. Scheier

University of Zurich - Division of Infectious Diseases and Hospital Epidemiology ( email )

Silvio D. Brugger

University of Zurich - Division of Infectious Diseases and Hospital Epidemiology ( email )

Raemistrasse 100
Zürich, 8091
Switzerland

Annelies S. Zinkernagel

University of Zurich - Division of Infectious Diseases and Hospital Epidemiology ( email )

Raemistrasse 100
Zürich, 8091
Switzerland

Pablo Sinues (Contact Author)

University Children’s Hospital Basel (UKBB) ( email )

Basel
Switzerland

Click here to go to Cell.com

Paper statistics

Downloads
12
Abstract Views
227
PlumX Metrics