Highly Selective Molecularly Imprinted Electrochemiluminescence Sensor Based on Perovskite/Ru(Bpy)32+ for Simazine Detection in Biological Samples

Abstract

A novel molecularly imprinted electrochemiluminescence (MIECL) sensor based on the luminescence of molecularly imprinted polymer-perovskite (MIP-CsPbBr 3 ) layer and Ru(bpy) 3 2+ was fabricated for simazine detection. MIP-CsPbBr 3 layers were immobilized onto the surface of glassy carbon electrode as the capture and signal amplification probe, and Ru(bpy) 3 2+ and co-reactant tripropylamine exhibited stronger electrochemiluminescence (ECL) emission. Under optimal conditions, the ECL signal of the MIECL sensor was linearly quenched, with the logarithm of simazine concentration ranging from 0.1 μg/L to 500.0 μg/L, correlation coefficient of 0.9947, and limit of detection of 0.06 μg/L. The practicality of the developed MIECL sensor method for simazine determination in biological samples was validated. Excellent recoveries of 86.5%–103.9% with relative standard deviation below 1.6% were obtained for fish and shrimp samples at three different spiked concentrations. The MIECL sensor exhibited excellent selectivity, sensitivity, reproducibility, accuracy, and precision for simazine determination in actual biological samples.

Keywords: Molecularly imprinted polymers, Perovskite, Ru(bpy)32+, Simazine, Electrochemiluminescence sensor