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Full-Length Structure of the Host Targeted Bacterial Effector Bep1 Reveals a Novel Structural Domain Conserved in FIC Effector Proteins From Bartonella

40 Pages Posted: 13 May 2022 Publication Status: Under Review

See all articles by Markus Huber

Markus Huber

University of Basel - Biozentrum

Alexander Wagner

University of Basel - Biozentrum

Jens Reiners

Heinrich Heine University Dusseldorf - Center for Structural Studies

Carsten Eric Maximilian Seyfert

University of Basel - Biozentrum

Timothy Sharpe

University of Basel - Biozentrum

Sander H.J. Smits

Heinrich Heine University Dusseldorf - Center for Structural Studies

Tilman Schirmer

University of Basel - Biozentrum

Christoph Dehio

University of Basel - Biozentrum

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Abstract

Bacterial effector proteins translocated via a type-IV secretion system (T4SS) typically harbor a C-terminal segment required for recognition by the type-IV secretion coupling protein. In the α-proteobacterial pathogen Bartonella, the signal is bipartite being composed of a BID (Bep intracellular delivery) domain and a positively charged C-terminal tail. Here, we show the crystal structure of full length Bartonella effector protein 1 (Bep1), which shows a FIC - OB - BAS(BID) domain arrangement conserved in the majority of Beps with the BID domain inserted into the newly discovered BAS parent domain. We propose that the BAS domain is necessary for the overall “boomerang”-like shape of Bep1 and that it plays a role during translocation through the T4SS.

Keywords: FIC, BEP, BID, Bartonella, Effector protein, type IV, secretion system, T4SS, VirB, VirD4, α-proteobacterial toxins

Suggested Citation

Huber, Markus and Wagner, Alexander and Reiners, Jens and Seyfert, Carsten Eric Maximilian and Sharpe, Timothy and Smits, Sander H.J. and Schirmer, Tilman and Dehio, Christoph, Full-Length Structure of the Host Targeted Bacterial Effector Bep1 Reveals a Novel Structural Domain Conserved in FIC Effector Proteins From Bartonella. Available at SSRN: https://ssrn.com/abstract=4109528 or http://dx.doi.org/10.2139/ssrn.4109528
This version of the paper has not been formally peer reviewed.

Markus Huber

University of Basel - Biozentrum ( email )

Alexander Wagner

University of Basel - Biozentrum ( email )

Jens Reiners

Heinrich Heine University Dusseldorf - Center for Structural Studies ( email )

Düsseldorf
Germany

Carsten Eric Maximilian Seyfert

University of Basel - Biozentrum ( email )

Timothy Sharpe

University of Basel - Biozentrum ( email )

Sander H.J. Smits

Heinrich Heine University Dusseldorf - Center for Structural Studies ( email )

Tilman Schirmer

University of Basel - Biozentrum ( email )

Christoph Dehio (Contact Author)

University of Basel - Biozentrum

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