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Correlation of the TCR Diversity Indexes in the Peripheral Blood with Disease Susceptibility and Deterioration in COVID-19 Patients
Guangxi University of Chinese Medicine (GXTCM) - Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine
Guangxi University of Chinese Medicine (GXTCM) - Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine
The 2019 coronavirus disease (COVID-19) caused by SARS-CoV-2 infection has become a global pandemic for more than two years. SARS-CoV-2 infection caused different clinical results, from none or mild symptoms to life threatening pneumonia. The determining factors for such discrepancy outcome are still not very clear. Accumulating studies suggest that the potency of the immune system in each individual has important influence on the clinical outcomes upon SARS-CoV-2 infection. Using high through put sequencing, we analyzed the expressed TCRβ repertoire in the peripheral blood of hospitalized mild and severe COVID-19 patients, cured COVID-19 patients and healthy donors. There are significant changes in the TCRβ repertoire upon viral infection compared to healthy donors and distinct features between mild and severe patients. All the COVID-19 patients had significantly lower TCR diversity indexes and significantly higher frequency of large oligo TCR clones compared to those in the healthy donors; the severe patients had significantly lower TCR diversity indexes and significantly higher frequencies of large oligo TCR clones compared to those in the mild patients. These results emphasize the significant role of T cells in anti-SARS-CoV-2 response and indicate that the TCR diversity index of each individual may be used as a novel predictor for disease susceptibility and deterioration upon SARS-CoV2 infection.
Funding Information: This study was supported by COVID-19 Emergency Research Fund from Sichuan(2020YFS0016, ZZ), Henan (No. 201100310900, YZ), and Guangxi (GuiKe AB20072005, JL) provinces. Sample preparation, high-through-put TCRβ sequencing, data analyses were performed by the Chengdu ExAb Biotechnology Ltd.
Declaration of Interests: ZZ is the founder of Chengdu ExAb Biotechnology Ltd. Other authors had no competing interests.
Ethics Approval Statement: All experiments using human samples were approved by the Ethics Committees of Sichuan Provincial Peoples Hospital (No: 2020-97), Fifth Medical Center of Chinese PLA General Hospital, The First Affiliated Hospital of Zhengzhou University, Henan Provincial People's Hospital, Zhoukou Infectious Disease Hospital, and The Fourth People's Hospital of Nanning. Informed consent was obtained from every participant.
Zhuo, Yue and Zhang, Ji-Yuan and Zhang, Zhen and Zhang, Lifeng and Yang, Xin and Song, Jin-Wen and Zhao, Xuan and Huang, Jianmin and Fan, Xing and Shuai, Ping and Wen, Xueping and Zhong, Xuemei and Xiao, Jian and Liu, Hong and Dai, Xiao-Peng and Xia, Peng and Xing, Lihua and Yao, Mengying and Wu, Qiuge and Liang, Hongxia and Zhu, Zhiqiang and Kang, Yi and Luo, XiaoLu and Liu, Zeduan and Wu, Yaofeng and Wang, Yunchao and Huang, Wanqiu and Wei, Liang and Huang, Xiaobo and Liu, Liping and Huang, Jian and Deng, Shaoping and Leng, Jing and Zhang, Yi and Wang, Fu-Sheng and Zhang, Zhixin, Correlation of the TCR Diversity Indexes in the Peripheral Blood with Disease Susceptibility and Deterioration in COVID-19 Patients (5/18/2022). Available at SSRN: https://ssrn.com/abstract=4115777 or http://dx.doi.org/10.2139/ssrn.4115777
Guangxi University of Chinese Medicine (GXTCM) - Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine
Guangxi University of Chinese Medicine (GXTCM) - Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine
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