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Hypoglycemic and Hypolipidemic Effects of Viburnum Foetidum (Linn.) Methanolic Extract in a Mice Model of Fructose Induced Type II Diabetes

27 Pages Posted: 9 Jun 2022 Publication Status: Preprint

See all articles by Iffat Hasnin

Iffat Hasnin

Rajshahi University - Department of Pharmacy

Sharmin Akter

Rajshahi University - Department of Pharmacy

Rafeza Khatun

Comilla University

Naznin Ara Khatune

Rajshahi University - Department of Pharmacy

Md. Mujahedul Islum

Rajshahi University - Department of Pharmacy

Aziz A. Rahman

Rajshahi University - Department of Pharmacy

Abstract

Aims: Viburnum foetidum (VF, family: Adoxaceae) is an evergreen plant widely distributed in the hilly areas of Bangladesh and is credited with various biological activities. The present study was designed to investigate in-vitro antioxidant and in-vivo hypoglycemic and hypolipidemic activity of methanolic extract of VF (CME) in fructose induced type II diabetic mice.

Main methods: The in-vitro antioxidant activity was conducted using standard biomedical methods for DPPH scavenging activity, iron reducing capacity and total antioxidant activity. Antidiabetic assay was performed by inducing diabetes in normal male Swiss Albino mice (30-40g) by feeding 10%-fructose solution for 7 weeks and the mice were divided into five group (n=5): NC (normal control), DC (diabetic control), DS (diabetic + Metformin 150 mg/kg/day), VF 200 and VF 400 (Diabetic + Extract 200 or 400 mg/kg/day for 15 days). The survival rate, changes in fasting blood glucose levels, OGTT, body weight, and lipid profile were recorded after treatment. SGOT, SGPT, antioxidant enzyme activities (SOD, CAT, MDA, GSH) of liver and histopathology of liver and pancreas were also evaluated.

Key findings: The methanolic extract of VF showed noticeable DPPH free radical scavenging (IC50 of 4.1 µg/ml) and iron reducing activity (1.47 µg/ml at 100 µg/ml concentration), and moderate total antioxidant activity as compared to standard indicating significant antioxidant capacity of the extract. The results of in-vitro antidiabetic activity revealed that oral administration of VF extracts significantly decreased the fasting blood sugar levels, TC, TG, LDL, VLDL, and significantly maintained body weight and the level of HDL as compared to the counterpart diabetic control mice (p<0.001 vs. DC). The level of SGPT and SGOT were significantly attenuated and enzymes activity of liver (increasing SOD, CAT and GSH and decreasing MDA level) was significantly restored which were comparable to normal control mice. The effects VF extracts on the protection of liver and pancreatic morphology was found by histopathological study. The effect of VF extract was dose dependent with most significant effect was observed at 400 mg/kg/day.

Significance: It may be inferred from the result that Viburnum foetidum may have potential to improve diabetes and associated hyperlipidemia as evidenced by lowering blood glucose level as well as decreasing cholesterol, TG and LDL and increasing antioxidant enzymatic activity (SOD, CAT, MDA, GSH) of liver.

Funding Information: The research was supported by Dean of science, University of Rajshahi, Rajshahi-6205, Bangladesh with grant number 1378/5/52/RU/Science-26/18-19 (dated: 20/12/2018).

Conflict of Interests: The authors declare that they have no competing interests.

Ethical Approval: The use and care of animals complied with the guidelines of the Institutional animal, medical ethics, biosafety and biosecurity committee (IAMEBBC) for experimentations on animal, human, microbes and living natural sources, University of Rajshahi, Bangladesh. Experimental procedures were approved by the “IAMEBBC” (Approval no: 37(21)/320/IAMEBBC/IBSc).

Keywords: Viburnum foetidum, Hypoglycemic and hypolipidemic activity, antioxidant activity, DPPH, Fructose induced diabetes

Suggested Citation

Hasnin, Iffat and Akter, Sharmin and Khatun, Rafeza and Khatune, Naznin Ara and Islum, Md. Mujahedul and Rahman, Aziz A., Hypoglycemic and Hypolipidemic Effects of Viburnum Foetidum (Linn.) Methanolic Extract in a Mice Model of Fructose Induced Type II Diabetes. Available at SSRN: https://ssrn.com/abstract=4132385 or http://dx.doi.org/10.2139/ssrn.4132385

Iffat Hasnin

Rajshahi University - Department of Pharmacy ( email )

Sharmin Akter

Rajshahi University - Department of Pharmacy ( email )

Rafeza Khatun

Comilla University ( email )

Comilla
Bangladesh

Naznin Ara Khatune

Rajshahi University - Department of Pharmacy ( email )

Md. Mujahedul Islum

Rajshahi University - Department of Pharmacy ( email )

Aziz A. Rahman (Contact Author)

Rajshahi University - Department of Pharmacy ( email )

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