Network Pharmacology Study on the Diuretic Effect of Plantaginis Semen and its Anti-Hypertensive Mechanism

26 Pages Posted: 10 Jun 2022

See all articles by chao li

chao li

Tianjin University of Traditional Chinese Medicine

Rou Wen

Jiangxi University of Traditional Chinese Medicine

DeWen Liu

China Academy of Chinese Medical Sciences - Institute of Chinese Materia Medica

Qiang Liu

Stanford University

LiPing Yan

Jiangxi University of Traditional Chinese Medicine

RuoNan Fan

Jiangxi University of Traditional Chinese Medicine

Qianfeng Gong

Jiangxi University of Traditional Chinese Medicine

Huan Yu

Jiangxi University of Traditional Chinese Medicine

Abstract

Background: Plantaginis Semen (PS), the mature seed of Plantago asiatica L. or Plantago depressa Willd., is a well-known Chinese medicine used as a diuretic and to remove dampness. It has been used to treat hypertension on account of its diuresis, but the efficacy of its diuresis is rarely reported. In addition, the systematic and holistic view of the anti-hypertensive mechanism of PS has not yet been elucidated.

Purpose: To assess diuretic effect of Plantaginis Semen and clarify its anti-hypertensive mechanism, promoting clinical applications of Plantaginis Semen to better treatment the patients.

Methods: Different doses of PS ethanol (PSE) extract were administered to rats in a saline-loaded model, and urine excretion and electrolytes in urine were used as indicators. In addition, the main components in PSE were identified through ultra-high-performance liquid chromatography, and then we further clarified the anti-hypertensive mechanism of PS, which may also its diuretic mechanism, through network pharmacology. Molecular docking of the hub gene and Western blot determination of key protein expression levels were performed.

Results: The results indicated that acteoside, isoacteoside, and plantamajoside may be the anti-hypertensive active ingredients of PS, and a dose-dependent effect was observed for PSE. The main metabolic pathways involved arachidonic acid metabolism, linoleic acid metabolism, and sphingolipid signaling pathways. This study will play a critical and guiding role in the dosing of PS as a diuretic drug for clinical applications. We also revealed the material foundation and mechanism of action that PS uses to regulate its anti-hypertensive effects through multi-target-multi-channel-multi-components, thereby providing a theoretical and scientific basis for the clinical application of PS.

Conclusion: PS with diuretic effect have therapeutic effects on hypertension by regulating RAAS, lipid metabolism, immune and inflammation related targets and pathways with the help of ingredients of phenylethanoid glycosides.

Note:

Funding Information: This work was supported by the National Standardization Project of Traditional Chinese 545 Medicine (ZYBZH-Y-JX-27) and National Natural Science Foundation of China (82160749) 546 (82104392).

Declaration of Interests: The authors declare no conflicts of interest.

Ethical Approval Statement: All of the experiments 139 were performed following the guidelines established by the Institutional Animal Care and Use 140 Committee of China and duly approved by the Animal Care and Research Committee of Jiangxi 141 University of Traditional Chinese Medicine (Nanchang, China) under license No. 142 JZLLSC2020_0108.

Keywords: Plantaginis Semen, diuretic, hypertension, network pharmacology, molecular docking

Suggested Citation

li, chao and Wen, Rou and Liu, DeWen and Liu, Qiang and Yan, LiPing and Fan, RuoNan and Gong, Qianfeng and Yu, Huan, Network Pharmacology Study on the Diuretic Effect of Plantaginis Semen and its Anti-Hypertensive Mechanism. Available at SSRN: https://ssrn.com/abstract=4133315 or http://dx.doi.org/10.2139/ssrn.4133315

Chao Li (Contact Author)

Tianjin University of Traditional Chinese Medicine ( email )

Tianjin
China

Rou Wen

Jiangxi University of Traditional Chinese Medicine ( email )

Nanchang
China

DeWen Liu

China Academy of Chinese Medical Sciences - Institute of Chinese Materia Medica ( email )

Qiang Liu

Stanford University ( email )

Stanford, CA 94305
United States

LiPing Yan

Jiangxi University of Traditional Chinese Medicine ( email )

Nanchang
China

RuoNan Fan

Jiangxi University of Traditional Chinese Medicine ( email )

Nanchang
China

Qianfeng Gong

Jiangxi University of Traditional Chinese Medicine ( email )

Nanchang
China

Huan Yu

Jiangxi University of Traditional Chinese Medicine ( email )

Nanchang
China

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