Development of an Automated Chemiluminescence Assay for Detection of Giardia lamblia in Canine Stool Samples

27 Pages Posted: 27 Jul 2022

Date Written: July 14, 2022

Abstract

This study introduces a novel immunoassay based on chemiluminescence detection and automation technologies. The Giardia Chemiluminescence Assay (GCA) detects Giardia antigens in a dose dependent manner with a limit of detection at 0.46 ng/ml and a dynamic range of 475-fold. A direct comparison between the GCA and a traditional ELISA using canine stool samples suggests that the GCA is at least 10-times more sensitive than ELISA in detecting Giardia antigens. To improve assay consistency and convenience, we transformed the manual GCA onto an automation platform, the ThunderBolt Analyzer. Automated GCA, while providing an assay result profile comparable with the manual assay, enables stringent step pacing and result precision, and it significantly reduces hands-on time for the assay operator. Automated GCA needs only an initial setup as the hands-on step in comparison with the manual assay that has 8 steps of hands-on operations spreading throughout the assay. In a study with clinic collected samples, canine stool samples are identified as Giardia positive or negative by a standard system Giardia II ELISA, and the GCA displays sensitivity at 93.8% and specificity at 92.9%. The GCA is the first system in the parasite diagnosis area to utilize chemiluminescence detection and full automation, and it is expected to improve work quality, especially for laboratories with medium to high volume tests.

Keywords: Automation, canine, chemiluminescence, ELISA, Giardia, immunoassay, parasites

Suggested Citation

Dong, Caixia and Liu, Ziye and Li, Xiaolin, Development of an Automated Chemiluminescence Assay for Detection of Giardia lamblia in Canine Stool Samples (July 14, 2022). Available at SSRN: https://ssrn.com/abstract=4163116 or http://dx.doi.org/10.2139/ssrn.4163116

Caixia Dong

GIMDx, Inc.

Ziye Liu

GIMDx, Inc.

Do you have a job opening that you would like to promote on SSRN?

Paper statistics

Downloads
22
Abstract Views
113
PlumX Metrics