Analysis of Notch1 Signaling in Mammalian Sperm Development

19 Pages Posted: 21 Jul 2022

See all articles by Naoto Sambe

Naoto Sambe

University of Tsukuba

Masaharu Yoshihara

University of Tsukuba

Teppei Nishino

University of Tsukuba

Ryosuke Sugiura

University of Tsukuba

Takahiro Nakayama

University of Tsukuba

Chandra Louis

University of Tsukuba

Satoru Takahashi

University of Tsukuba - Laboratory Animal Resource Center

Abstract

Highlights● The Delta-Notch signaling component Hes1 was expressed in Sertoli cells but not in the sperm cell lineage.● A transgenic reporter mouse study revealed no Notch1 signal in the sperm cell lineage.● Single-cell RNA sequencing of testes from mice and humans revealed Notch1 expression in Sertoli cells but not in sperm cells.

Keywords: Cre/loxP system, Delta-Notch signaling pathway, Gal4-UAS system, single-cell RNA-sequencing, testis

Suggested Citation

Sambe, Naoto and Yoshihara, Masaharu and Nishino, Teppei and Sugiura, Ryosuke and Nakayama, Takahiro and Louis, Chandra and Takahashi, Satoru, Analysis of Notch1 Signaling in Mammalian Sperm Development. Available at SSRN: https://ssrn.com/abstract=4169064 or http://dx.doi.org/10.2139/ssrn.4169064

Naoto Sambe

University of Tsukuba ( email )

Tsukuba University , Ibaraki Ken
Tsukuba, Ibaraki 305-8573, 3050006
Japan

Masaharu Yoshihara (Contact Author)

University of Tsukuba ( email )

Tsukuba University , Ibaraki Ken
Tsukuba, Ibaraki 305-8573, 3050006
Japan

Teppei Nishino

University of Tsukuba ( email )

Tsukuba University , Ibaraki Ken
Tsukuba, Ibaraki 305-8573, 3050006
Japan

Ryosuke Sugiura

University of Tsukuba ( email )

Tsukuba University , Ibaraki Ken
Tsukuba, Ibaraki 305-8573, 3050006
Japan

Takahiro Nakayama

University of Tsukuba ( email )

Tsukuba University , Ibaraki Ken
Tsukuba, Ibaraki 305-8573, 3050006
Japan

Chandra Louis

University of Tsukuba ( email )

Tsukuba University , Ibaraki Ken
Tsukuba, Ibaraki 305-8573, 3050006
Japan

Satoru Takahashi

University of Tsukuba - Laboratory Animal Resource Center ( email )

Do you have a job opening that you would like to promote on SSRN?

Paper statistics

Downloads
26
Abstract Views
190
PlumX Metrics