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Humoral Immunity and Transcriptome Differences of COVID-19 Inactivated Vaccine and Protein Subunit Vaccine as Third Booster Dose in Human

30 Pages Posted: 28 Jul 2022

See all articles by Yuwei Zhang

Yuwei Zhang

Shandong Center for Disease Control and Prevention

Mingxiao Yao

Shandong Center for Disease Control and Prevention

Xingyu Guo

Shandong First Medical University - School of Public Health

Shanshan Han

Shandong First Medical University - School of Public Health

Shu Zhang

Shandong Center for Disease Control and Prevention

Jinzhong Zhang

Liaocheng Center for Disease Control and Prevention

Xiangkun Jiang

Liaocheng Center for Disease Control and Prevention

Jianxing Wang

Shandong Center for Disease Control and Prevention

Ming Fang

Shandong Center for Disease Control and Prevention

Shuang Wang

Shandong Center for Disease Control and Prevention

Bo Pang

Shandong Center for Disease Control and Prevention

Xiaolin Liu

Shandong Center for Disease Control and Prevention

Zengqiang Kou

Shandong Center for Disease Control and Prevention

Xiaolin Jiang

Shandong Center for Disease Control and Prevention

More...

Abstract

Background: Against the background of the severe human health and world economic burden caused by COVID-19, the attenuation of vaccine protection efficacy, and the prevalence of variants of concern (VOCs), the third dose of booster immunization has been put on the agenda.

Methods: Systems biology approaches can help us gain new perspectives on the characterization of immune responses and the identification of factors underlying vaccine-induced immune efficacy. We analyzed the antibody signature and transcriptional responses of participants vaccinated with SARS-CoV-2 inactivated vaccine and protein subunit vaccine as a third booster dose.

Findings: The results from the antibody indicated that the third booster dose was effective, and that sequential immunization with the protein subunit vaccine as a booster dose induced stronger humoral immune responses than the inactivated vaccine, and might be more effective against VOCs. In transcriptomic analysis, protein subunit vaccine induced more differentially expressed genes that were significantly associated with many important innate immune pathways.

Interpretation: Both the homologous and heterologous boosters could increase the effectiveness against COVID-19, and compared with the inactivated vaccine, the protein subunit vaccine, mediated a stronger humoral immune response and had a more significant correlation with the innate immune function module, which provided certain data support for the sequential immunization strategy.

Funding Information: This work was supported by the Major Scientific and Technological Innovation Project in Shandong Province (grant numbers: 2020SFXGFY02-1) and Key Research and Development plan of Shandong Province (grant numbers: 2021RZA01021).

Declaration of Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Ethics Approval Statement: This study was approved by the Ethical Approval Committee of Shandong Center for Disease Control and Prevention.

Keywords: COVID-19, Third booster vaccine, Variants of concern, Humoral immunity, Transcriptome analysis

Suggested Citation

Zhang, Yuwei and Yao, Mingxiao and Guo, Xingyu and Han, Shanshan and Zhang, Shu and Zhang, Jinzhong and Jiang, Xiangkun and Wang, Jianxing and Fang, Ming and Wang, Shuang and Pang, Bo and Liu, Xiaolin and Kou, Zengqiang and Jiang, Xiaolin, Humoral Immunity and Transcriptome Differences of COVID-19 Inactivated Vaccine and Protein Subunit Vaccine as Third Booster Dose in Human. Available at SSRN: https://ssrn.com/abstract=4175229 or http://dx.doi.org/10.2139/ssrn.4175229

Yuwei Zhang

Shandong Center for Disease Control and Prevention ( email )

Mingxiao Yao

Shandong Center for Disease Control and Prevention ( email )

Xingyu Guo

Shandong First Medical University - School of Public Health ( email )

Shanshan Han

Shandong First Medical University - School of Public Health ( email )

Shu Zhang

Shandong Center for Disease Control and Prevention ( email )

Jinzhong Zhang

Liaocheng Center for Disease Control and Prevention ( email )

Xiangkun Jiang

Liaocheng Center for Disease Control and Prevention ( email )

Jianxing Wang

Shandong Center for Disease Control and Prevention ( email )

Ming Fang

Shandong Center for Disease Control and Prevention ( email )

Shuang Wang

Shandong Center for Disease Control and Prevention ( email )

Bo Pang

Shandong Center for Disease Control and Prevention ( email )

Xiaolin Liu

Shandong Center for Disease Control and Prevention ( email )

Zengqiang Kou

Shandong Center for Disease Control and Prevention ( email )

Xiaolin Jiang (Contact Author)

Shandong Center for Disease Control and Prevention ( email )

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