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A Luciferase Fragment Complementation Assay to Detect Focal Adhesion Kinase (FAK) Signaling Events

63 Pages Posted: 5 Aug 2022 Publication Status: Under Review

See all articles by Jason A. Estep

Jason A. Estep

University of California Riverside

Lu O. Sun

University of Texas at Dallas - The University of Texas Southwestern Medical Center

Martin Riccomagno

University of California Riverside

Abstract

Integrin Adhesion Complexes (IACs) serve as links between the cytoskeleton and extracellular environment, acting as mechanosensing and signaling hubs. As such, IACs participate in many aspects of cellular motility, tissue morphogenesis, anchorage-dependent growth and cell survival. Focal Adhesion Kinase (FAK) has emerged as a critical organizer of IAC signaling events due to its early recruitment and diverse substrates, and thus has become a genetic and therapeutic target. Here we present the design and characterization of simple, reversible, and scalable Bimolecular Complementation sensors to monitor FAK phosphorylation in living cells. These probes provide novel means to quantify IAC signaling, expanding on the currently available toolkit for interrogating FAK phosphorylation during diverse cellular processes.

Keywords: Integrin Adhesion Complexes, Focal Adhesions, Focal Adhesion Kinase, Integrin, Cell Migration, Cell Motility, Luciferase Fragment Complementation Assay, Split Luciferase

Suggested Citation

Estep, Jason A. and Sun, Lu O. and Riccomagno, Martin, A Luciferase Fragment Complementation Assay to Detect Focal Adhesion Kinase (FAK) Signaling Events. Available at SSRN: https://ssrn.com/abstract=4182286 or http://dx.doi.org/10.2139/ssrn.4182286

Jason A. Estep

University of California Riverside ( email )

900 University Avenue
Riverside, CA 92521
United States

Lu O. Sun

University of Texas at Dallas - The University of Texas Southwestern Medical Center ( email )

TX
United States

Martin Riccomagno (Contact Author)

University of California Riverside ( email )

900 University Avenue
Riverside, CA 92521
United States

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