Structural Characterization Of Arabinogalactan from Stauntonia Leucantha Fruits

Abstract

Polysaccharides were the key ingredients of many herbal medicines, and were reponsible for multiple pharmacological activities. In this study, a novel polysaccharide fraction, named SLP-2, was isolated from  Stauntonia leucantha  fruits, and purified by DEAE-52 and Sephadex G-100 column chromatograph. Furthermore, SLP-2 was identified by congo red, methylation, partial acid hydrolysis and NMR. The results indicated that the backbone of SLP-2 was composed of →4)-β-D-Gal p -(1→4)-β-D-Gal p -(1→ substituted at C-6 with 1,5-linked arabinan. SLP-2 had good anti-oxidation ability in vitro . Surprisingly, we found that reduction of carboxyl groups and methylation of hydroxyl groups enhanced the ability to scavenge ABTS radicals and inhibit lipid peroxidation, and weakened the activity to scavenge DPPH radicals and reduce ferric iron.

Keywords: Stauntonia leucantha, Polysaccharides, structural characterization, Antioxidant activity