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The Role of Electrons’ Spin in DNA Oxidative Damage Recognition

21 Pages Posted: 16 Sep 2022 Publication Status: Published

See all articles by Qirong Zhu

Qirong Zhu

Weizmann Institute of Science - Department of Chemical and Biological Physics

Yael Kapon

Hebrew University of Jerusalem - Department of Applied Physics

Aaron M. Fleming

University of Utah - Department of Chemistry

Suryakant Mishra

Weizmann Institute of Science - Department of Chemical and Biological Physics

Kakali Santra

Weizmann Institute of Science - Department of Chemical and Biological Physics; University of Groningen - Centre for Systems Chemistry

Francesco Tassinari

Weizmann Institute of Science - Department of Chemical and Biological Physics

Sidney R. Cohen

Weizmann Institute of Science - Chemical Research Support

Tapan Kumar Das

Weizmann Institute of Science - Department of Chemical and Biological Physics

Yutao Sang

Weizmann Institute of Science - Department of Chemical and Biological Physics

Deb Kumar

Weizmann Institute of Science - Department of Chemical and Biological Physics

Cynthia J. Burrows

University of Utah - Department of Chemistry

Yossi Paltiel

Hebrew University of Jerusalem - Department of Applied Physics

Ron Naaman

Weizmann Institute of Science - Department of Chemical and Biological Physics

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Abstract

Formation of 8-oxo-7,8-dihydro-2’-deoxyguanosine (OG) is one of the most common forms of DNA oxidative damage found in human cells. Although this damage is prevalent in many disease states, it only marginally influences the structure and stability of double-stranded DNA (dsDNA). Therefore, it is a challenge to establish the mechanism by which this damage is detected by repair enzymes. We investigated the position-dependent effect of the damage on the interactions between dsDNA and oligopeptides using atomic force microscopy. The results were confirmed by monitoring the spin and location-dependent polarizability of the damaged DNA, applying a Hall device. The observations suggest that the interaction of peptide with DNA depends on oxidative damage in the DNA and on its location relative to the point of contact between the peptide and the DNA. Hence, a remote search mechanism for damage in DNA is possible.

Keywords: DNA, damage, polarization, spin, oligopeptide

Suggested Citation

Zhu, Qirong and Kapon, Yael and Fleming, Aaron M. and Mishra, Suryakant and Santra, Kakali and Tassinari, Francesco and Cohen, Sidney R. and Das, Tapan Kumar and Sang, Yutao and Kumar, Deb and Burrows, Cynthia J. and Paltiel, Yossi and Naaman, Ron, The Role of Electrons’ Spin in DNA Oxidative Damage Recognition. Available at SSRN: https://ssrn.com/abstract=4220599 or http://dx.doi.org/10.2139/ssrn.4220599
This version of the paper has not been formally peer reviewed.

Qirong Zhu

Weizmann Institute of Science - Department of Chemical and Biological Physics ( email )

Yael Kapon

Hebrew University of Jerusalem - Department of Applied Physics ( email )

Aaron M. Fleming

University of Utah - Department of Chemistry ( email )

Suryakant Mishra

Weizmann Institute of Science - Department of Chemical and Biological Physics ( email )

Kakali Santra

Weizmann Institute of Science - Department of Chemical and Biological Physics

University of Groningen - Centre for Systems Chemistry

P.O. Box 800
9700 AH Groningen, Groningen 9700 AV
Netherlands

Francesco Tassinari

Weizmann Institute of Science - Department of Chemical and Biological Physics ( email )

Sidney R. Cohen

Weizmann Institute of Science - Chemical Research Support

Tapan Kumar Das

Weizmann Institute of Science - Department of Chemical and Biological Physics ( email )

Yutao Sang

Weizmann Institute of Science - Department of Chemical and Biological Physics ( email )

Deb Kumar

Weizmann Institute of Science - Department of Chemical and Biological Physics ( email )

Cynthia J. Burrows

University of Utah - Department of Chemistry ( email )

Yossi Paltiel

Hebrew University of Jerusalem - Department of Applied Physics ( email )

Ron Naaman (Contact Author)

Weizmann Institute of Science - Department of Chemical and Biological Physics ( email )

Israel

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