Morphological and Molecular Confirmation of Ornithodoros Hasei (Schulze, 1935) (Acari: Argasidae) in Colombia

14 Pages Posted: 7 Oct 2022

See all articles by Paula A. Ossa-López

Paula A. Ossa-López

University of Caldas

Lorys Y. Mancilla-Agrono

University of Caldas

Lizeth Fernanda Banguero Micolta

University of Caldas

Héctor E. Ramírez-Chaves

University of Caldas

Juan David Carvajal Agudelo

University of Caldas

Sebastián Muñoz-Leal

University of Concepción - Departamento de Ciencia Animal

Marcelo B. Labruna

University of São Paulo (USP) - Department of Preventive Veterinary Medicine and Animal Health

Vett Lloyd

Mount Allison University

Fredy A. Rivera-Páez

Universidad de Caldas

Abstract

A large number of tick species are proven vectors for the transmission of bacteria, protozoa and viruses. Soft ticks (Acari: Argasidae) in South America have been found to be the most frequent carriers of Relapsing fever group Borrelia (RFGB); however, there are several information gaps specially on the taxonomy and distribution of some species. Here, we used light microscopy, scanning electron microscopy and PCR amplification of a fragment of the mitochondrial 16S rRNA gene to evaluate 174 larvae of Ornithodoros (Argasidae) collected from three bat species (Eptesicus orinocensis, Molosssus rufus and Noctilio albiventris) in the Orinoquia Region of Colombia. The morphological and molecular results confirmed that all the analyzed larvae corresponded to Ornithodoros hasei. Comparisons of mitochondrial 16S rDNA sequences showed low genetic divergence (0% - 0.3%) between larvae of the Department of Arauca in the Orinoquia Region and higher genetic divergence (3.4-4.7%) in sequences from other American countries. Our work represents the most recent collection of this species in Colombia and gives a molecular evaluation for the first time. Moreover, a new association of O. hasei with bats such as E. orinocensis is documented. Considering the wide distribution of O. hasei in the American Continent, and its putative role as vector for Borrelia sp., integrative studies that involve morphological, morphometric, molecular data and experimental crosses are needed to determine if the higher genetic distances are associated with cryptic speciation, as detected in other tick complexes, or represent genetic divergences among geographically different populations of O. hasei.

Keywords: Arauca, Borrelia, Chiroptera, Mammalia, Orinoquia Region, Soft Ticks

Suggested Citation

Ossa-López, Paula A. and Mancilla-Agrono, Lorys Y. and Micolta, Lizeth Fernanda Banguero and Ramírez-Chaves, Héctor E. and Agudelo, Juan David Carvajal and Muñoz-Leal, Sebastián and Labruna, Marcelo B. and Lloyd, Vett and Rivera-Páez, Fredy A., Morphological and Molecular Confirmation of Ornithodoros Hasei (Schulze, 1935) (Acari: Argasidae) in Colombia. Available at SSRN: https://ssrn.com/abstract=4240833

Paula A. Ossa-López

University of Caldas ( email )

Colombia

Lorys Y. Mancilla-Agrono

University of Caldas ( email )

Colombia

Lizeth Fernanda Banguero Micolta

University of Caldas ( email )

Colombia

Héctor E. Ramírez-Chaves

University of Caldas ( email )

Colombia

Juan David Carvajal Agudelo

University of Caldas ( email )

Colombia

Sebastián Muñoz-Leal

University of Concepción - Departamento de Ciencia Animal ( email )

Marcelo B. Labruna

University of São Paulo (USP) - Department of Preventive Veterinary Medicine and Animal Health ( email )

Vett Lloyd

Mount Allison University ( email )

Sackville, EOA 3CO
United States

Fredy A. Rivera-Páez (Contact Author)

Universidad de Caldas ( email )

Manizales
Colombia

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