Structural Characterization and Bifidogenic Activity of Polysaccharides from Dictyophora Indusiata

Abstract

Dictyophora indusiata is an edible and medicinal fungi with various nutritious, especially higher in carbohydrates. This study aims to investigate the structural characterization and bifidogenic activity of polysaccharides from Dictyophora indusiate (DIP). In this work, four polysaccharide fractions (DIP0, DIP1, DIP2 and DIP3) were isolated from Dictyophora indusiata by cellulose DE-52 column. Structural properties of four DIP were characterized by HPLC, FT-IR and SEM. Four polysaccharide fractions were composed of mannose, glucose and galactose with almost the same functional groups, while DIP3 additionally contained glucuronic acid. In addition, DIP0 exhibited a three-dimensional network structure formed by crosslinked chains in space, while DIP1, DIP2 and DIP3 displayed the irregular flake structure. Notably, DIP0, DIP1 and DIP2 could obviously stimulate the growth of Bifidobacterium longum, and DIP0 and DIP1 promoted the production of acetic acid after 36 h fermentation in vitro. Our findings provided necessary information on the prebiotic of DIP.

Keywords: Dictyophora indusiate, polysaccharides, Structural characterization, Bifidobacterium, acetic acid