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Isolation and Characterization of Crude-Oil-Dependent Bacteria from the Coast of Ghana Using Oxford Nanopore Sequencing

33 Pages Posted: 19 Dec 2022 Publication Status: Published

See all articles by Mark Kwasi Sarfo

Mark Kwasi Sarfo

Ghana Atomic Energy Commission

Samuel Gyasi

University of Energy & Natural Resources

Amos Tiereyangn Kabo-bah

University of Energy and Natural Resources (UENR) - Department of Civil and Environmental Engineering

Bright Adu

University of Ghana

Quaneeta Mohktar

University of Ghana

Andrew Sarkodie Appiah

Ghana Atomic Energy Commission

Yaw Serfor-Armah

University of Ghana

Abstract

The utilization and improper use of crude oil have had irreparable damage on the environment and human populations. This study sought to isolate hydrocarbon utilizing bacteria from 1% v/v pristine seawater and 1% v/v crude oil using enrichment methods. Whole genome sequencing of DNA using the Oxford Nanopore sequencing technique with Fastq WIMP as the workflow at 3% abundance was undertaken. The results showed that the most abundant isolates identified using this technique at specific sampling sites were, Acinetobacter junii (51.9%), Alcanivarax pacificus (15.8%), Acinetobacter haemolyticus (21.6%), Pseudomonas aeruginosa (23.4%), Alcanivorax xenomutans (24.7%), Alcanivorax xenomutans (23.0%) Acinetobacter baumannii (40.0%) and Acinetobacter junii (14.2%). Cumulatively, the most abundant isolates in the 8 sampling sites were Acinetobacter junii (17.91%), Alcanivorax xenomutans (11.68%), Pseudomonas aeruginosa (7.68%), Escherichia coli (7.67%), Acinetobacter haemolyticus (3.40%), and Alkanivorax pacificus (3.10%). Spearman’s rank correlation analysis to examine the strength of relationship between the physicochemical parameters and type of bacteria isolated, revealed that salinity (0.8046) and pH (0.7252) were the highest. Isolated bacteria from pristine seawater, especially Escherichia coli have shown their capacity for bioremediating oil spill pollution in oceanic environments in Ghana.

Keywords: Jubilee oil, Coast of Ghana, hydrocarbon, bacteria, Sequencing

Suggested Citation

Sarfo, Mark Kwasi and Gyasi, Samuel and Kabo-bah, Amos Tiereyangn and Adu, Bright and Mohktar, Quaneeta and Appiah, Andrew Sarkodie and Serfor-Armah, Yaw, Isolation and Characterization of Crude-Oil-Dependent Bacteria from the Coast of Ghana Using Oxford Nanopore Sequencing (December 9, 2022). Available at SSRN: https://ssrn.com/abstract=4297898 or http://dx.doi.org/10.2139/ssrn.4297898

Mark Kwasi Sarfo (Contact Author)

Ghana Atomic Energy Commission ( email )

P. O. Box LG80
Legon-Accra
Ghana

Samuel Gyasi

University of Energy & Natural Resources ( email )

Amos Tiereyangn Kabo-bah

University of Energy and Natural Resources (UENR) - Department of Civil and Environmental Engineering

Sunyani, +233
Ghana

Bright Adu

University of Ghana

Quaneeta Mohktar

University of Ghana ( email )

Andrew Sarkodie Appiah

Ghana Atomic Energy Commission ( email )

P. O. Box LG80
Legon-Accra
Ghana

Yaw Serfor-Armah

University of Ghana ( email )

PO Box 25
Legon, LG
Ghana

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