Synchrotron-Based X-Ray Fluorescence Microscopy Mapping the Ionome of a Toxic Freshwater Cyanobacterium

Abstract

Harmful algal blooms (HABs) pose a major environmental concern across the globe. In abundance, cyanobacteria, or so-called green-blue algae can produce extremely dangerous cyanotoxins that harm humans and animals. This study focused on the mapping and distribution of intracellular macro-and micronutrients of the wide-spread freshwater cyanobacteria Microcystis aeruginosa (M. aeruginosa) and the effect on their photosynthetic activity, cell metabolism and cyanotoxin production at the cellular and organelle level. Towards a better understanding of trace metal uptake and homeostasis throughout the cell cycle, we quantitatively mapped the spatial distribution of the elements P, K, Fe, Ca, Zn, Mn, and Cu across the ultrastructure of frozen-hydrated single cells using state-of-the-art X-ray nanofluorescence imaging at the Advanced Photon Source (APS) at Argonne National Laboratory. Bulk cellular nutrient and trace metal content correlated well with the total intracellular elemental content in individual cells obtained by quantitative synchrotron X-ray fluorescence measurements. Multi-dimensional mappings showed P and K atoms colocalized as discrete semicircular hotspots that were analyzed with respect to their stoichiometry. Elevated Cu and Ca concentrations were detected along division plane of cells. P and K were found to have similar spatial elemental distribution with about 65% and 69% of the total cellular P and K, respectively, located at the hotspots. The P and K colocalization were refined further using nanotomography, showing a K envelope surrounding the P core. Inorganic P and organic P compounds were specified using solution-state 31P nuclear magnetic resonance (NMR) spectroscopy from M. aeruginosa. Of the total P determined by 31P NMR, 47% were found to be nucleotides while only 11% were polyphosphates. Multimodal X-ray imaging provides a better understanding of intracellular biochemical processes in cyanobacteria, helping us monitor and combat an emerging environmental threat.

Keywords: Elemental mapping, Cyanobacteria, 31P NMR spectroscopy, Nanotomography, Synchrotron X-ray Fluorescence