Detection of Escherichia Coli and Other Enterobacteriales Members in Seabirds Sampled Along the Brazilian Coast

70 Pages Posted: 24 Jan 2023

See all articles by Maira Duarte Cardoso

Maira Duarte Cardoso

Oswaldo Cruz Foundation (FIOCRUZ) - National School of Public Health Sergio Arouca

Verônica Dias Gonçalves

Fundação Oswaldo Cruz - Instituto Oswaldo Cruz

Andrea Soffiatti Grael

UFF - Universidade Federal Fluminense

Vanessa Marques Pedroso

Universidade Federal do Rio Grande (FURG)

Jeferson Rocha Pires

Estácio de Sá University

Carlos Eurico Pires Ferreira Travassos

State University of Northern Rio de Janeiro

Camila Domit

Federal University of Paraná

Olney Vieira-Da-Motta

State University of Northern Rio de Janeiro

Dália dos Prazeres Rodrigues

Fundação Oswaldo Cruz - Instituto Oswaldo Cruz

Salvatore Siciliano

Fundação Oswaldo Cruz - Escola Nacional de Saúde Pública

Abstract

Escherichia coli, an Enterobacterales member, is a normal representative of the microbiota of warm-blooded animals. Most strains are commensal, but several pathotypes can cause disease, and numerous antimicrobial resistance factors have been identified. These bacteria have spread rapidly in recent years, highlighting the importance of screening the environment and non-human reservoirs for virulent strains and/or those presenting resistance factors, in addition to other microorganisms of public health importance. In this context, this study aimed to survey Enterobacteriales present in seabirds sampled along the Brazilian coast, comparing findings between migratory and resident birds, as well as between wrecked and non-wrecked animals. E. coli pathotypes were also characterized through rapid seroagglutination and polymerase chain reaction techniques and antimicrobial resistance profiles were investigated through the disc agar diffusion method. Cloacal, ocular, oral, tracheal, and skin lesion swabs, as well as fresh feces, were collected from 122 seabirds. The findings indicate these animals as important hosts for opportunistic human pathogens. E. coli strains were identified in 70% of the analyzed seabirds, 62% of which displaying resistant or intermediate profiles to at least one antimicrobial, while 7% were multiresistant. Resistance to tetracycline (22%), nalidixic acid (15%), trimethoprim-sulfamethozaxol (14%) and ampicillin (12%) were the most prevalent. Resistance to cefoxitin, a critically important antimicrobial for human medicine, was also detected. Virulence genes for one of the EAEC, ETEC or EPEC pathotypes were detected in 30% of the identified strains, the first two described in seabirds for the first time. The EAEC gene was detected in 25% of the sampled seabirds, all resident, 8% of which exhibited a multidrug-resistant profile. Thus, seabirds comprise important reservoirs for this pathotype. E. coli was proven an ubiquitous and well-distributed bacterium, present in all evaluated bird species and sampling sites. According to the chi-square test, no significant differences between E. coli prevalences or antimicrobial resistance profiles between migratory and resident and between wrecked and non-wrecked seabirds were observed. Thus, migratory birds do not seem to contribute significantly to E. coli frequencies, pathotypes or antimicrobial resistance rates on the Brazilian coast.

Keywords: Escherichia Coli, Seabirds, Enterobacteriales, Pathotypes, Antimicrobial Resistance, EAEC, ETEC, EPEC

Suggested Citation

Cardoso, Maira Duarte and Gonçalves, Verônica Dias and Grael, Andrea Soffiatti and Pedroso, Vanessa Marques and Pires, Jeferson Rocha and Travassos, Carlos Eurico Pires Ferreira and Domit, Camila and Vieira-Da-Motta, Olney and Rodrigues, Dália dos Prazeres and Siciliano, Salvatore, Detection of Escherichia Coli and Other Enterobacteriales Members in Seabirds Sampled Along the Brazilian Coast. Available at SSRN: https://ssrn.com/abstract=4333080 or http://dx.doi.org/10.2139/ssrn.4333080

Maira Duarte Cardoso (Contact Author)

Oswaldo Cruz Foundation (FIOCRUZ) - National School of Public Health Sergio Arouca ( email )

Verônica Dias Gonçalves

Fundação Oswaldo Cruz - Instituto Oswaldo Cruz ( email )

Andrea Soffiatti Grael

UFF - Universidade Federal Fluminense ( email )

Vanessa Marques Pedroso

Universidade Federal do Rio Grande (FURG) ( email )

Rio Grande
Brazil

Jeferson Rocha Pires

Estácio de Sá University ( email )

Carlos Eurico Pires Ferreira Travassos

State University of Northern Rio de Janeiro ( email )

Camila Domit

Federal University of Paraná ( email )

Olney Vieira-Da-Motta

State University of Northern Rio de Janeiro ( email )

Dália dos Prazeres Rodrigues

Fundação Oswaldo Cruz - Instituto Oswaldo Cruz ( email )

Salvatore Siciliano

Fundação Oswaldo Cruz - Escola Nacional de Saúde Pública ( email )

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