Peroxidase-Like Phosphate Hydrate Nanosheets Bio-Synthesized by a Marine Shewanella algae Strain for Highly Sensitive Dopamine Detection

Abstract

The sensitive and rapid detection of dopamine (DA) is of great significance for early diagnosis of related diseases. Current detection strategies of DA are time-consuming, expensive and inaccurate, while biosynthetic nanomaterials were considered highly stable and environment friendly, which were promising on colorimetric sensing. Thus in this study, novel zinc phosphate hydrate nanosheets (SA@ZnPNS) biosynthesized by Shewanella algae were designed for the detection of DA. SA@ZnPNS showed high peroxidase-like activity which catalyzed the oxidation reaction of 3,3',5,5'-tetramethylbenzidine in the presence of H2O2. Results showed that the catalytic reaction of SA@ZnPNS followed Michaelis-Menton kinetics and ping-pong mechanism with the chief active species of hydroxyl radical. The colorimetric detection of DA in human serum samples was performed based on SA@ZnPNS peroxidase-like activity. The linear range of DA detection was 0.1-40 μM, and the detection limit was 0.083 μM. This study provided a simple and practical method for the detection of DA and expanded the application of biosynthesized nanoparticles to biosensing fields.

Keywords: Shewanella algae, Green biosynthesis, Dopamine, Colorimetric detection, Zinc phosphate hydrate nanoparticles