Deterioration of Muscle Quality Caused by Ammonia Exposure in Rainbow Trout (Oncorhynchus Mykiss)

27 Pages Posted: 18 Feb 2023

See all articles by Yiwen Wu

Yiwen Wu

Hubei Academy of Agricultural Sciences

Manman Zhao

Hubei Academy of Agricultural Sciences

Yuting Xia

Hubei Academy of Agricultural Sciences

Weiqing Sun

Yangtze University

Guangquan Xiong

Hubei Academy of Agricultural Sciences

Liu Shi

Hubei Academy of Agricultural Sciences

Yu Qiao

Hubei Academy of Agricultural Sciences

Wenjing Wu

Hubei Academy of Agricultural Sciences

Anzi Ding

Hubei Academy of Agricultural Sciences

Lang Chen

Hubei Academy of Agricultural Sciences

Lan Wang

Hubei Academy of Agricultural Sciences

Sheng Chen

Hubei Academy of Agricultural Sciences

Abstract

In order to explore deterioration of muscle quality caused by ammonia exposure in rainbow trout (Oncorhynchus mykiss), and then preliminarily investigating the regulatory mechanism of muscle quality in O. mykiss after ammonia exposure based on the lipid metabolism, different concentrations of ammonia stress experiment were conducted, and the physicochemical properties of muscle, antioxidant system, products of energy and lipid metabolism in the muscle of rainbow trout were investigated at total ammonia concentration of 0 (control group), 10 mg/L (T1), 20 mg/L (T2), 30 mg/L (T3), 45 mg/L (T4) and 60 mg/L (T5). In comparison with the control group, the fillets after ammonia stress exhibited higher a* value, expressible moisture, essential amino acids (EAA), the bitter amino acid and mono-saturated fatty (MUFA), less the umami and sweet amino acids and polyunsaturated fatty acid (PUFA), worse flavor and taste. The lower ammonia exposure (lower than 30 mg/L) did not change the content of EPA and DHA while significant decreases were observed after higher ammonia exposure (higher than 30 mg/L). Meanwhile, the lower ammonia exposure activated the antioxidant system and higher ammonia exposure inactivated antioxidant enzymes to defend against reactive oxygen species (ROS). Moreover, the expression of d9D, FAS, LPL, PPARa, PPARy and SREBP were inhibited and the expression of CPT1 was promoted after ammonia stress, inhibiting lipid synthesis and promoting lipid oxidation. The results are important in establishing the relationship among muscle quality, lipid metabolism and the environmental adaptability of rainbow trout (Oncorhynchus mykiss).

Keywords: ammonia exposure, rainbow trout fillets, muscle quality, free amino acid, fatty acid

Suggested Citation

Wu, Yiwen and Zhao, Manman and Xia, Yuting and Sun, Weiqing and Xiong, Guangquan and Shi, Liu and Qiao, Yu and Wu, Wenjing and Ding, Anzi and Chen, Lang and Wang, Lan and Chen, Sheng, Deterioration of Muscle Quality Caused by Ammonia Exposure in Rainbow Trout (Oncorhynchus Mykiss). Available at SSRN: https://ssrn.com/abstract=4363148 or http://dx.doi.org/10.2139/ssrn.4363148

Yiwen Wu

Hubei Academy of Agricultural Sciences ( email )

China

Manman Zhao

Hubei Academy of Agricultural Sciences ( email )

China

Yuting Xia

Hubei Academy of Agricultural Sciences ( email )

China

Weiqing Sun

Yangtze University ( email )

China

Guangquan Xiong

Hubei Academy of Agricultural Sciences ( email )

China

Liu Shi

Hubei Academy of Agricultural Sciences ( email )

China

Yu Qiao

Hubei Academy of Agricultural Sciences ( email )

China

Wenjing Wu

Hubei Academy of Agricultural Sciences ( email )

China

Anzi Ding

Hubei Academy of Agricultural Sciences ( email )

China

Lang Chen

Hubei Academy of Agricultural Sciences ( email )

China

Lan Wang

Hubei Academy of Agricultural Sciences ( email )

China

Sheng Chen (Contact Author)

Hubei Academy of Agricultural Sciences ( email )

China

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