How to Scramble? A 'Wise Way' to Achieve a Useful Control Peptide Sequence

Abstract

“Scrambled peptide” is a well-established concept in the literature and ubiquitously used where biological or functional activity of peptides is studied. It is regularly created via random shuffling of the original sequence and normally used as the synonym of homologous negative control peptide. However, random shuffling may result in a molecule with totally different spatial or charge distribution than the original. Also, may inherit active sequence motifs, spoiling the goal, to reach a negative control. Our study is about the development of an application to overcome these issues, for free of charge.

Instead of trying to force a single definition and recipe, our application is flexible, designed to optimize the scrambling process and improve the feasibility of control peptides, which we labeled as wise shuffling.

Keywords: scrambled peptides, bioactive, control, application, permutation