Overexpression of Atbzip69 Gene Confers Tolerance to Nitrogen Stress and Increases Field Yield in Transgenic Wheat

13 Pages Posted: 6 Mar 2023

See all articles by Jiji Yan

Jiji Yan

Shanxi Normal University

Daoping Wang

affiliation not provided to SSRN

Shuyu Kang

Shanxi Normal University

Zhang He

Harbin Normal University

Xin Li

affiliation not provided to SSRN

Huawei Shi

affiliation not provided to SSRN

Wensi Tang

affiliation not provided to SSRN

Kai Chen

affiliation not provided to SSRN

Yinghong Pan

affiliation not provided to SSRN

Yongbin Zhou

affiliation not provided to SSRN

Zhaoshi Xu

affiliation not provided to SSRN

Jun Chen

affiliation not provided to SSRN

Youzhi Ma

affiliation not provided to SSRN

Ming Chen

affiliation not provided to SSRN

Chunhua Pang

Shanxi Normal University

Abstract

Nitrogen is an important nutrients of growth and yield in wheat, however, the excessive application of fertilizer has exerted a great threat on the environment. Therefore, it is crucial to enhanced nitrogen use efficiency (NUE), but only few related genes have been researched in field crops. In this study, we generated wheat plants showing improved low nitrogen (LN) tolerance, by introducing AtbZIP69, a gene encoding a bZIP transcription factor, into Shi 4056. We found that AtbZIP69 in transgenic wheat was induced by LN and drought. AtbZIP69 is localized in the nucleus and activates transcription. The greenhouse trial revealed that compared to the wild type (WT), the AtbZIP69 transgenic lines showed significantly increased drought and LN stress tolerance. A two-year field trial demonstrated that the yield and number of spikes of transgenic lines was significantly higher than the WT under LN conditions. By investigating N-use-related traits, we discovered that overexpression of AtbZIP69 altered N distribution by allocating more N to grains under LN conditions. In addition, the expression of N transporter protein genes (e.g. TaNRT2.1, TaNRT2.5, TaNR1) was higher in AtbZIP69 transgenic lines than in WT under LN. Overall, AtbZIP69 overexpression increased the nitrogen content of transgenic lines grains under LN conditions by regulating the expression of nitrogen-related transporter protein genes, potentially leading to increased grain yield. As a result, a new candidate gene for improving LN stress tolerance in the field was identified with the use of wheat heterologous expression analysis.

Keywords: bZIP transcription factors, low nitrogen stress, nitrogen use efficiency, grain yield, transgenic wheat

Suggested Citation

Yan, Jiji and Wang, Daoping and Kang, Shuyu and He, Zhang and Li, Xin and Shi, Huawei and Tang, Wensi and Chen, Kai and Pan, Yinghong and Zhou, Yongbin and Xu, Zhaoshi and Chen, Jun and Ma, Youzhi and Chen, Ming and Pang, Chunhua, Overexpression of Atbzip69 Gene Confers Tolerance to Nitrogen Stress and Increases Field Yield in Transgenic Wheat. Available at SSRN: https://ssrn.com/abstract=4379681 or http://dx.doi.org/10.2139/ssrn.4379681

Jiji Yan

Shanxi Normal University ( email )

Lifen
China

Daoping Wang

affiliation not provided to SSRN ( email )

No Address Available

Shuyu Kang

Shanxi Normal University ( email )

Lifen
China

Zhang He

Harbin Normal University ( email )

Harbin
China

Xin Li

affiliation not provided to SSRN ( email )

No Address Available

Huawei Shi

affiliation not provided to SSRN ( email )

No Address Available

Wensi Tang

affiliation not provided to SSRN ( email )

No Address Available

Kai Chen

affiliation not provided to SSRN ( email )

No Address Available

Yinghong Pan

affiliation not provided to SSRN ( email )

No Address Available

Yongbin Zhou

affiliation not provided to SSRN ( email )

No Address Available

Zhaoshi Xu

affiliation not provided to SSRN ( email )

No Address Available

Jun Chen

affiliation not provided to SSRN ( email )

No Address Available

Youzhi Ma

affiliation not provided to SSRN ( email )

No Address Available

Ming Chen

affiliation not provided to SSRN ( email )

No Address Available

Chunhua Pang (Contact Author)

Shanxi Normal University ( email )

Lifen
China

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