Effects of Ifosfamide and Cisplatin Exposure Combined with a Climate Change Scenario on the Transcriptome Responses of the Mussel Mytilus Galloprovincialis

26 Pages Posted: 11 Mar 2023

See all articles by Vanessa Queirós

Vanessa Queirós

affiliation not provided to SSRN

Ulisses M. Azeiteiro

affiliation not provided to SSRN

Marta Casado

affiliation not provided to SSRN

Juan Luis Santos

University of Seville

Esteban Alonso

University of Seville

Amadeu M.V.M. Soares

Universidade de Aveiro - Department of Biology

Rosa Freitas

Universidade de Aveiro - Department of Biology

Benjamin Piña

affiliation not provided to SSRN

Carlos Barata

Institute of Environmental Assessment and Water Research (IDAEA-CSIC)

Abstract

Coastal ecosystems are currently exposed to pollutants and climate changes. Namely, the increasing consumption of antineoplastics drugs and their potential release to aquatic ecosystems are raising concerns. Nevertheless, information regarding the toxicity of these drugs towards non-target species is scarce, especially considering climate change scenarios. Ifosfamide (IF) and cisplatin (CDDP) are among the antineoplastics already detected in aquatic compartments and due to their mode of action (MoA) can negatively affect aquatic organisms. Thia study evaluates the transcription of target genes related to the MoA of IF and CDDP in Mytillus galloprovincialis exposed to environmental relevant low concentrations of these drugs, under an actual (17 ºC) and predicted warming scenario (21 ºC). Results showed an upregulation of the cyp4y1 gene when exposed to the highest concentrations of IF, regardless of the temperature. Both drugs upregulated genes related to DNA damage and apoptosis (p53, caspase 8 and gadd45), especially in warmer conditions. Increased temperature also downregulated genes related to stress and immune responses (krs and mydd88). Therefore, the present results showed that antineoplastics combined with temperature alterations can affect transcriptomic responses of M. galloprovincialis, by changing their sensitivity towards these drugs.

Keywords: Anticancer drugs, Bivalves, Transcriptomic, Genetic expression, climate change

Suggested Citation

Queirós, Vanessa and Azeiteiro, Ulisses M. and Casado, Marta and Santos, Juan Luis and Alonso, Esteban and Soares, Amadeu M.V.M. and Freitas, Rosa and Piña, Benjamin and Barata, Carlos, Effects of Ifosfamide and Cisplatin Exposure Combined with a Climate Change Scenario on the Transcriptome Responses of the Mussel Mytilus Galloprovincialis. Available at SSRN: https://ssrn.com/abstract=4385615 or http://dx.doi.org/10.2139/ssrn.4385615

Vanessa Queirós

affiliation not provided to SSRN ( email )

No Address Available

Ulisses M. Azeiteiro

affiliation not provided to SSRN ( email )

No Address Available

Marta Casado

affiliation not provided to SSRN ( email )

No Address Available

Juan Luis Santos

University of Seville ( email )

Avda. del Cid s/n
Sevilla, 41004
Spain

Esteban Alonso

University of Seville ( email )

Avda. del Cid s/n
Sevilla, 41004
Spain

Amadeu M.V.M. Soares

Universidade de Aveiro - Department of Biology ( email )

Rosa Freitas

Universidade de Aveiro - Department of Biology ( email )

Benjamin Piña

affiliation not provided to SSRN ( email )

No Address Available

Carlos Barata (Contact Author)

Institute of Environmental Assessment and Water Research (IDAEA-CSIC) ( email )

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