Biomass Production of C. Pyrenoidosa by Filled Sphere Carrier Reactor: Performance and Mechanism

Abstract

Microalgae-based Carbon Capture, Utilization and Storage is one of the key technologies to cope with global climate change. This study developed a filled sphere carrier reactor, expecting to achieve high C. pyrenoidosa biomass production by optimizing key operational parameters. Results showed that the five-fold concentrated nutrient solution combining with the addition of 0.2 mol/L phosphate buffer effectively avoided significant pH fluctuations and improved critical functional enzymes activities. Under the optimized conditions, the wet biomass yield reached up to 220.2 g/L/d. Compared with the conventional suspension culture, the wet biomass was enhanced as high as 39.45 times. The mechanism was mainly attributed to the obvious intensification of electron transfer rate and remarkable increase of RuBisCO enzyme activity in the photosynthetic chloroplast matrix. Therefore, this work provided a novel filled sphere carrier reactor for potential high C. pyrenoidosa biomass production.

Keywords: C. pyrenoidosa, Filled sphere carrier reactor, High biomass production, electron transfer