Infection of Epithelioma Papulosum Cyprini (EPC) Cells With Spring Viremia of Carp Virus (SVCV) Induces Autophagy and Apoptosis Through Endoplasmic Reticulum Stress

15 Pages Posted: 18 May 2023

See all articles by Xue Jiao

Xue Jiao

Jilin Agricultural University (JLAU)

Yu-ting Lu

Jilin Agricultural Science and Technology University

Aidong Qian

Jilin Agricultural University (JLAU) - College of Animal Science and Technology

Yue-Hong Li

Jilin Agricultural University (JLAU)

Abstract

Spring viremia of carp virus (SVCV) is a lethal freshwater pathogen of cyprinid fish that has caused significant economic losses to aquaculture. To reduce the economic losses caused by SVCV, its pathogenic mechanism needs to be studied more thoroughly. Here, we report for the first time that SVCV infection of Epithelioma papulosum cyprini (EPC) cells can induce cellular autophagy and apoptosis through endoplasmic reticulum stress. The presence of autophagic vesicles in infected EPC cells was shown by transmission electron microscopy. Quantitative fluorescence PCR and Western blot results showed that p62 mRNA expression was decreased, and the expression of Beclin1 and LC3 mRNA was increased. The p62 protein was decreased, and the Beclin1 protein and LC3 were increased in the endoplasmic reticulum stress activation state. To further clarify the mode of death of SVCV-infected EPC cells, we examined caspase3, caspase9, BCL-2, and Bax mRNA, which showed that they were all increased. Apoptosis of SVCV-infected cells increased upon activation of endoplasmic reticulum stress. Our results suggest that endoplasmic reticulum stress can regulate SVCV infection-induced autophagy and apoptosis. The results of this study provide theoretical data for the pathogenesis of SVCV and lay the foundation for future drug development and vaccine construction.

Keywords: Spring viremia of carp virus (SVCV), Autophagy, Apoptosis, Epithelioma papulosum cyprinid (EPC) cells, Endoplasmic reticulum stress.

Suggested Citation

Jiao, Xue and Lu, Yu-ting and Qian, Aidong and Li, Yue-Hong, Infection of Epithelioma Papulosum Cyprini (EPC) Cells With Spring Viremia of Carp Virus (SVCV) Induces Autophagy and Apoptosis Through Endoplasmic Reticulum Stress. Available at SSRN: https://ssrn.com/abstract=4441677 or http://dx.doi.org/10.2139/ssrn.4441677

Xue Jiao

Jilin Agricultural University (JLAU) ( email )

Yu-ting Lu

Jilin Agricultural Science and Technology University

Aidong Qian

Jilin Agricultural University (JLAU) - College of Animal Science and Technology ( email )

China

Yue-Hong Li (Contact Author)

Jilin Agricultural University (JLAU) ( email )

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