Synergistic Effect of Synthesized Zno Nanoflowers Coupled with Various Antibiotics Against Pathogenic Microbes: Characterization, Antibacterial and Antifungal Activity Assessment

24 Pages Posted: 16 May 2023

See all articles by Makaoui Nassima

Makaoui Nassima

affiliation not provided to SSRN

Nassiba Moghni

University Saad Dahlab Blida

Boutemak khalida

affiliation not provided to SSRN

imoudache hicham

affiliation not provided to SSRN

Akkache Lynda

affiliation not provided to SSRN

Hadj-Ziane-Zafour Amel

affiliation not provided to SSRN

Abstract

Zinc oxide nanoparticles (denoted as ZnO NPs) were synthesized via a simple sol-gel technique using only two precursors. Various techniques were utilized to characterize the prepared ZnO nanoparticles. The X-ray diffraction analysis indicated the formation of nanoparticulated crystallite ZnO wheras the XPS spectra confirrmed the occurance of Zn and O atoms. SEM imaging showed nanoflower morphology with varying particle sizes at the nanoscale; whereas DLS characterization revealed a mean particle diameter that equals 19 nm. The EDX technique confirmed the presence of Zn and O with 85.43% and 12.4% respectively. Meantime the adopted colorimetric method revealed a 99.9% ZnO loading implying the high purity of the synthesized material. The synergetic effect of combining ZnO nanoflowers with antibiotics, namely chlortetracycline hydrochloride, spiramycin, and azithromycin on enhancing the antibacterial activity against various bacterial strains was investigated via disc diffusion technique. The antifungal activity of the synthesized ZnO-antibiotic was also examined. The antibacterial and antifungal activity of chlortetracycline hydrochloride, spiramycin enhanced in the presence of ZnO nanomaterial confirming the synergistic impact of the ZnO-chlortetracycline and ZnO-spiramycin conjunctions. The ZnO-CTC materials exhibed the best synergistic effect both against gram-negative and gram-positive organisms. The inhibitory zone of ZnO-CTC in Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, as well as  Escherichia coli plates were 19.6, 14.0, 17.8, and 18.6 % higher than that of CTC alone. The inhibition zone of ZnO-CTC against Candida albicans, Saccharomyces cereviciae, and Aspergillus brasiliensis incresead by 42.4%, 21.0%, and 21.6% in comapraison to bare ZnO, respectively. This implies the combination of ZnO-CTC outperforms ZnO in terms of antibacterial efficiency. On the other hand, the antibacterial and antifungal efficiencies were decreased when coupling ZnO with azithromycin antibiotic implying that pairing it with azithromycin had a no effect.The obtained findings confirm that the synthesized ZnO-antibiotic material could be utilized in combination with different antibiotics as a powerful alternate antibacterial and antifungal agent against highly pathogenic resistant species.

Keywords: ZnO, nanoflowers, antibiotics, Antibacterial activity, Antifungal activity, Synergistic effect.

Suggested Citation

Nassima, Makaoui and Moghni, Nassiba and khalida, Boutemak and hicham, imoudache and Lynda, Akkache and Amel, Hadj-Ziane-Zafour, Synergistic Effect of Synthesized Zno Nanoflowers Coupled with Various Antibiotics Against Pathogenic Microbes: Characterization, Antibacterial and Antifungal Activity Assessment. Available at SSRN: https://ssrn.com/abstract=4450453 or http://dx.doi.org/10.2139/ssrn.4450453

Makaoui Nassima

affiliation not provided to SSRN ( email )

No Address Available

Nassiba Moghni

University Saad Dahlab Blida ( email )

Boutemak Khalida

affiliation not provided to SSRN ( email )

No Address Available

Imoudache Hicham

affiliation not provided to SSRN ( email )

No Address Available

Akkache Lynda

affiliation not provided to SSRN ( email )

No Address Available

Hadj-Ziane-Zafour Amel (Contact Author)

affiliation not provided to SSRN ( email )

No Address Available

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