Cga Alleviates Lps-Induced Inflammation and Milk Fat Reduction in Bmecs Through the Nf-Κb Signaling Pathway

Abstract

Mastitis is a common clinical disease in dairy cows that seriously reduces the yield and quality of milk and seriously affects animal health. Chlorogenic acid (CGA) is a polyphenolic substance rich in Eucommia ulmoides leaves that has anti-inflammatory effects and improves oxidative stress. Here, we investigated whether CGA attenuated lipopolysaccharide(LPS)-induced inflammation and decreased milk fat in bovine mammary epithelial cells(BMECs). To construct a mastitis model use 10 µg/mL LPS to stimulate the BMECs. The effects of CGA on the inflammatory response, oxidative stress, and milk fat synthesis of BMECs were measured by qRT‒PCR, Western blotting, oil red O staining, and triglyceride (TG) determination. The results showed that CGA alleviated LPS-induced inflammation in BMECs, reduced the expression of inflammatory factors such as IL-6, IL-8, TNF-α, IL1-β, and INOS, reduced the oxidative stress level of cells, increased the gene expression levels of CHOP, XCT, NRF2, and HO-1, and alleviated the decrease in milk fat synthesis. In addition, we found that CGA may exert its anti-inflammatory and antioxidant effects through the NF-κB signaling pathway. Our study showed that CGA could alleviate LPS-induced inflammation and oxidative stress in BMECs by regulating the NF-κB signaling pathway and rescuing the decrease in milk fat.

Keywords: Chlorogenic acid, mastitis, BMECs, oxidative stress, NF-κB